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| Montalván,Ricardo; Ando,Akihiko; Echeverrigaray,Sérgio. |
| Grain proteins from 58 Brazilian and nine Japanese upland rice cultivars (Oryza sativa L.) were electrophoretically separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric scanning of the electrophoretic profiles permitted the estimation of the relative concentration of 16 protein fractions, which were used as variables for the calculation of Fisher's canonical discriminating functions. Significant differences between mean values of protein fractions were useful in distinguishing Brazilian and Japanese cultivars, as well as improved and non-improved Brazilian rice cultivars in scattered plots. Electrophoretically detectable protein polymorphism in rice grain can indicate geographic origin as well as breeding... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47571998000400021 |
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| Cansian,Rogério Luis; Mossi,Altemir José; Mazutti,Márcio; Oliveira,José Vladimir; Paroul,Natalia; Dariva,Cláudio; Echeverrigaray,Sérgio. |
| The use of compressed carbon towards extracting semi-volatile compounds present in maté leaves (Ilex paraguariensis St. Hil.) is due to the growing interest in mate constituents to develop new products in the cosmetic, pharmaceutical and food industries. The objective of this work was to assess the chemical distribution of semi-volatile compounds in 20 native populations of maté collected all over Brazil. The extracts of bulk samples (30 plants) of each population were obtained by the high-pressure carbon dioxide extraction technique, and analyzed by GC/MSD. The quantification of compounds (caffeine, theobromine, phytol, squalene, vitamin E, eicosane, pentatriacontane, and stigmasterol) showed significant variations within the different populations and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Maté; Chemical variability; Brazilian native populations; Supercritical extraction. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100021 |
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| Wollheim,Claudia; Guerra,Ivani Maria F; Conte,Vania D; Hoffman,Sheila P; Schreiner,Fernando J; Delamare,Ana Paula L; Barth,Afonso L; Echeverrigaray,Sérgio; Costa,Sérgio Olavo P da. |
| OBJECTIVES: To determine the prevalence of class A extended spectrum β-lactamases (ESBL)-producing Escherichia coli and Klebsiella spp., and to investigate clonality among ESBL-producing isolates of nosocomial and community infections. METHODS: The study involved 354 nosocomial infections samples and 992 community infections samples, obtained between 2003 and 2006 at Caxias do Sul, RS. The detection of ESBL was performed by the disk-diffusion test. Presence of blaCTX-M, blaSHV and blaTEM β-lactamase genes was evaluated by PCR, and genomic typing was determined by pulsed-field gel electrophoresis analysis. RESULTS: Higher frequency of ESBL-producing isolates were detected among nosocomial samples of E. coli (6.7%) and Klebsiella (43.7%), than those obtained... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-lactamases; Polymerase chain reaction; Bacterial typing techniques. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200008 |
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| Michelim,Lessandra; Lahude,Michele; Araújo,Patricia R.; Giovanaz,Dirce S. H.; Müller,Gabriela; Delamare,Ana P.L.; Costa,Sérgio Olavo Pinto da; Echeverrigaray,Sérgio. |
| Nosocomial infections constitute an important problem in hospitals, intensive care units (ICU) having the highest incidence of this type of infection. Staphylococci, especially Staphylococcus epidermidis and Staphylococcus aureus, are among the most important microorganisms associated with nosocomial infections. S. epidermidis is a common skin resident, and can be introduced into the clinical environment by patients and hospital staff. The situation in hospitals is aggravated by the emergence of multiresistant strains. We evaluated 98 hospital S. epidermidis isolates collected at neonatal, pediatric and adult ICUs and 20 S. epidermidis control skin resident isolates from healthy volunteers, for resistance to ten antibiotics and chemotherapeutic agents, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus epidermidis; Pathogenicity factors; Antibiotic resistance; Hospital infection. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100004 |
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