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| Pozza,M.; Simonetti,A.B.; Esteves,P.A.; Rijsewijk,F.A.M.; Roehe,P.M.. |
| Empregou-se a técnica de reação em cadeia pela polimerase precedida de transcrição reversa para detecção do vírus da cinomose canina (CC). Para a padronização da técnica foram selecionados quatro pares de oligonucleotídeos (P1, P2, N1, H1), baseados em seqüências dos genes da fosfoproteína, neuraminidase e hemaglutinina, sendo utilizadas três cepas vacinais de vírus da CC como controles positivos. Foram analisadas três amostras isoladas de cães com cinomose e quatro amostras provenientes de cães com suspeita clínica de cinomose. Não houve amplificação nas amostras com suspeita clínica da doença. Os resultados obtidos com os oligonucleotídeos P1 e N1 foram superiores aos de H1. Os oligonucleotídeos P2 foram considerados inapropriados para a detecção do... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cão; Cinomose; RT-PCR; Polimorfismo. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000500010 |
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| Ritterbusch,G.A.; Esteves,P.A.; Trevisol,I.M.; Okino,C.H.; Jaenisch,F.R.F.; Morés,M.A.Z.; Caron,L.; Silva,A.D.; Finger,P.F.; Hübner,S.O.. |
| RESUMO A vacinação é a forma mais utilizada para prevenir a bronquite infecciosa causada pelo vírus da bronquite infecciosa das galinhas (IBV). Contudo, as vacinas convencionais são incapazes de diferenciar aves infectadas de vacinadas. No presente trabalho foi construído, caracterizado, e avaliado como candidato vacinal, um adenovírus recombinante expressando o gene N do IBV. O gene N foi clonado em um adenovírus humano tipo 5 defectivo e transfectado para as células HEK-293A para gerar rAd5_N. Após o vetor ser obtido como esperado e a confirmação da expressão da proteína N em HEK-293ª, foi realizada inoculação pela via oculo-nasal na dose de 10 7 TCID 50 /0,1mL para imunização de galinhas livres de patógenos específicos (SPF). A resposta imunológica do... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Infectious bronchitis virus; Nucleocapsid protein; Adenovirus vector; Immune responses. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000401428 |
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| Spilki,F.R.; Silva,T.C.; Esteves,P.A.; Teixeira,M.B.; Batista,H.B.C.R.; Chiminazzo,C.; Driemeier,D.; Franco,A.C.; Roehe,P.M.. |
| During a series of experiments attempting to reproduce clinically apparent bovine herpesvirus type 5 (BoHV-5) infections, a group of calves was inadvertently infected with bovine viral diarrhoea virus (BVDV). Another group of calves was infected with BoHV-5 only. This paper reports the outcome of such infections. Two out of six calves solely infected with BoHV-5 displayed moderate to severe clinical signs. Three out of four calves of the group co-infected with BoHV-5 and BVDV developed severe clinical signs, two of them died. BoHV-5 virus was isolated to higher titres and for a longer period from the group of calves infected with both viruses. These results suggest that BVDV may enhance clinical signs induced by BoHV-5 and may also play a role in extending... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-5; BVDV; Co-infection; Experimental inoculation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000500001 |
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| Vasconcelos,J.; Soliman,M.C.; Staggemeier,R.; Heinzelmann,L.; Weidlich,L.; Cimirro,R.; Esteves,P.A.; Silva,A.D.; Spilki,F.R.. |
| Hepatitis E virus (HEV) is highly disseminated among swine herds worldwide. HEV is also a threat to public health, since particularly genotypes 3 and 4 may cause acute hepatitis in human beings. No previous studies were done on the occurrence of HEV in environmental samples in Rio Grande do Sul, Brazil. In the present study, reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect the presence of HEV in swine feces and in effluents from slurry lagoons in farms located in the municipality of Teutônia, inside the area of swine husbandry in the state. Pooled fecal samples from the floor of pig barns from 9 wean-to-finish farms and liquid manure samples were collected from the slurry lagoons from 8 of these farms. From the pooled fecal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis E virus; Swine HEV; Slurry; Zoonosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300777 |
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| Puentes,R; Eliopulos,N; Pérez,R; Franco,G; Sosa,K; Bianchi,P; Furtado,A; Hübner,S.O.; Esteves,P.A.. |
| Canine parvovirus type 2 (CPV-2) is a leading cause of diarrhea in puppies in several parts of the world. In this study CPV-2 was detected and recovered from puppies showing clinical disease from Montevideo, Uruguay. Samples were processed and used to infect CRFK and MDCK cells in order to isolate the virus. Out of twelve, two samples were positive for CPV-2. A genomic region of 583 bp was amplified and the molecular characterization was performed by sequencing, phylogenetic analysis and Restriction Fragment Length Polymorphism (RFLP). Two isolated viruses (UY1 and UY2) were CPV-2c-like viruses. The comparison between the cytophatic effect (CPE) of CPV-2 (vaccinal virus) and CPV-2c (isolated virus) on primary canine cells cultures and on CRFK line cells,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine Parvovirus 2c; Isolation; Molecular characterization; South America. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300022 |
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