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	Provedor de dados Electron. J. Biotechnol. (2) Autor Etcheverrigaray,Marina (2) Kratje,Ricardo (2) Bollati Fogolín,Mariela (1) Eberhardt,Marcos Oggero (1) Forno,Guillermina (1) Marini,Guillermo (1) Mais... Palavra-chave Glycosylation (1) Pharmacokinetic parameters (1) RhGM-CSF (1) Tipo do documento Journal article (2) Ano 2007 (1) 2002 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Recombinant human granulocyte-macrophage colony-stimulating factor: effect of glycosylation on pharmacokinetic parameters Electron. J. Biotechnol. Marini,Guillermo; Forno,Guillermina; Kratje,Ricardo; Etcheverrigaray,Marina. The pharmacokinetic behaviour of the non-glycosylated, bacterially-derived recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) and the glycosylated mammalian product was studied after intra and extra vascular administration of a single dose in rodents. Each route of administration gave a different rhGM-CSF concentration-time profile. After extra vascular administration of equivalent doses, a higher peak concentration and faster elimination were observed in the group treated with the E. coli-derived cytokine. The faster elimination resulted in a return to pre-treatment plasma levels after 12 hrs, versus 48 hrs following the administration of glycosylated rhGM-CSF. After intravascular administration, clearance of rhGM-CSF was... Tipo: Journal article Palavras-chave: Glycosylation; Pharmacokinetic parameters; RhGM-CSF. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200011 Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems Electron. J. Biotechnol. Bollati Fogolín,Mariela; Eberhardt,Marcos Oggero; Kratje,Ricardo; Etcheverrigaray,Marina. Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein content, and therefore useful for determining concentrations of the cytokine in production processes. Among these assays, the competitive ELISA, developed with a MAb that recognises an epitope not involved in glycosylation, was the only one suitable to quantify the glycosylated form of rhGM-CSF produced in mammalian cell cultures. Tipo: Journal article Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582002000300008 Registros recuperados: 2 Primeira ... 1 ... Última

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