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| Colas, Florent; Tardivel, Morgan; Evrard, Justine; Forest, Bertrand; Crassous, Marie-pierre; Lunven, Michel; Danielou, Marie-madeleine. |
| This deliverable contains all development work of the FastCAM, new tool for fast imaging of the phytoplankton, which took place in 2015. It consists of: One report of the FastCAM and its comparison with the FlowCAM presentation: The FlowCAM enables the digitalization of a sample of phytoplankton with X10 and X 4 magnifications. The first allows a better morphological description and therefore a higher taxonomic resolution but with a longer scan time (16 X). Thus, for routine analyses, only scanning at 4 X is possible. A considerable gain would be able to do an acquisition to the magnification 10 X with a time of analysis more short, comparable to 4 X. A fast flow imaging system has therefore developed in this sense. He was called FastCAM. The system is... |
| Tipo: Text |
Palavras-chave: FlowCAM; FastCAM; Imagerie rapide; Phytoplancton; REPHY.; FlowCAM; FastCAM; Fast imaging; Phytoplankton; REPHY. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00363/47420/47425.pdf |
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| Blondel, Marc; Soubigou, Flavie; Evrard, Justine; Phu Hai Nguyen,; Hasin, Naushaba; Chedin, Stephane; Gillet, Reynald; Contesse, Marie-astrid; Friocourt, Gaelle; Stahl, Guillaume; Jones, Gary W.; Voisset, Cecile. |
| 6AP and GA are potent inhibitors of yeast and mammalian prions and also specific inhibitors of PFAR, the protein-folding activity borne by domain V of the large rRNA of the large subunit of the ribosome. We therefore explored the link between PFAR and yeast prion [PSI+] using both PFAR-enriched mutants and site-directed methylation. We demonstrate that PFAR is involved in propagation and de novo formation of [PSI+]. PFAR and the yeast heat-shock protein Hsp104 partially compensate each other for [PSI+] propagation. Our data also provide insight into new functions for the ribosome in basal thermotolerance and heat-shocked protein refolding. PFAR is thus an evolutionarily conserved cell component implicated in the prion life cycle, and we propose that it... |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00358/46931/46834.pdf |
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