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| Scheffer, K. C.; Fahl, W. O.; Iamamoto, K.; Carnieli Jr., P.; Carrieri, M. L.; Oliveira, R. N.; Ito, F. H.. |
| Molecular techniques have been used increasingly as tools for the diagnosis by detecting the rabies virus genome. This study aimed to detect the presence of rabies virus in the wash of skull and in different organs of the genus Artibeus bats using the hemi-nested RT-PCR (hnRT-PCR) and Real Time RT-PCR molecular techniques. From approximately 4,000 specimens of bats received at the Institute Pasteur for rabies diagnosis, 30 bats of the genus Artibeus were selected, with records of positive results for rabies by the traditional techniques of direct fluorescent antibody test (FAT) and inoculation of murine neuroblastoma cell line (N2A). Salivary glands, urinary bladders, kidneys, lungs, and also the washes of the skullcaps of the specimens were collected. The... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3105 |
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| Carnieli Jr., P.; Batista, H. B.C.R.; Schereffer, K. C.; Fahl, W. O.; Lima, J. Y.O.; Oliveira, R. N.; Castilho, J. G.; Iamamoto, K.; Carrieri, M. L.; Kotait, I.. |
| The identification of animal species that transmit pathogens such as the rabies virus is of the utmost importance for public health and the natural history of infectious and contagious diseases. Diagnostic laboratories very often receive mauled or decomposing animal carcasses, particularly of bats, rendering morphometric identification unviable. The existence of different regional names for the same animal, morphological variability and the lack of staff trained in zoological identification constitute a serious problem for epidemiological surveillance. Molecular techniques are used routinely and effectively in systematics, evolution and ecology to identify species and can even be used to identify hybrids that originated from genetically close animals, in... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3106 |
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| Ikeda, M. A. K.; Barros, R. F.; Lima, J. Y. O.; Ferreira, J. S.; Pereira, P. M. C.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| The bats are a major reservoir of rabies virus and have a relevant importance in the disease transmission. The aim of this study was to evaluate the bat population submitted for rabies diagnosis. Data were analyzed from 18.805 bats received by the diagnostic section of the Pasteur Institute of Sao Paulo, originated from several counties of the state of Sao Paulo over the period from 2007 to 2012. These specimens were morphologically classified according to their family, at the time of execution of the rabies diagnosis technique. The central nervous system from these animals was submitted to direct immunofluorescence test and, when viable, to viral isolation. From the total of bats received, 76.92% bats belonged to the Molossidae family, 12.14% to the... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17383 |
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| Mori, E.; Scheffer, K. C.; Fahl, W. O.; Oliveira, R. N.; Asano, K. M.; Carnieli Jr, P.; Carrieri, M. L.; Kotait, I.. |
| Human rabies is still an important public health problem in some Brazilian regions. Usually, ante-mortem diagnosis of rabies is made by demonstration of virus antigen by direct immunofluorescence in corneal or conjunctival smears and skin biopsies; however, this technique has a low sensitivity. Recently, molecular techniques such as the reverse transcriptase polymerase chain reaction (RT-PCR) and nucleic acid sequence based amplification assay (NASBA) have been developed to improve the sensitivity and specificity of ante as well as post-mortem diagnosis of rabies. Rapid and accurate diagnosis of ante-mortem human rabies is essential for effective medical management and to ensure appropriate post-exposure prophylaxis of potential contacts with the patient.... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3098 |
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| Pereira, P. M. C.; Lima, J. Y. O.; Barros, R. F.; Ferreira, J. S.; Ikeda, M. A. K.; Fahl, W. O.; Iamamoto, K.; Mori, E.; Asano, K. M.; Ferreira, E.; Achkar, S. M.; Scheffer, K. C.. |
| Bats found in Brazil are classified in 9 families, 64 genus and 168 species. They are an important rabies reservoirs and all species can be infected or be the transmitter of rabies virus, independently of their specie classification or feeding habits. This work aimed a survey of bats species originated from counties of Sao Paulo state, which were sent to the diagnostic laboratory of the Pasteur Institute – Sao Paulo. Between August 2009 to October 2012; 9.109 bats specimens were received and submitted to recommended techniques for rabies diagnosis by World Health Organization. From these specimens, 8.421 (92,45%) were classified in family, genus and specie, by morphological and morphometric parameters using bats identification keys. Furthermore, they also... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17386 |
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| Scheffer, K. C.; Fahl, W. O.; Lamamoto, K.; Carnieli Junior, P.; Carrieri, M. L.; Mori, E.; Oliveira, R. N.; Ito, F. H.. |
| The aim of this study was to detect the rabies virus (RABV) presence in different organs of frugivorous bats using molecular techniques such as RT‐PCR, hnRT‐PCR, and the Real Time RT‐PCR. Thirty bats of the genus Artibeus were selected and resulted as positive by the DFA test and N2A‐cells inoculation test using brain tissue in both tests. Samples of salivary gland tissue, urinary bladder tissue, kidney tissue, lung tissue, stool, and skull lavage were collected for molecular assays. The organs and the stool were diluted at 1:10 (w/v) and the urinary bladder was diluted at 1:20 (w/v). The RT‐PCR and the hnRT‐PCR were performed using specific nucleoprotein gene‐target primers. The product obtained by reverse transcription technique was submitted to the Real... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17385 |
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| Jarcovis, R. L. M.; Gamon, T. H. M.; Nogi, K. I.; Fahl, W. O.; Asano, K. M.; Achkar, S. M.; Maiorka, P. C.; Mori, C. M. C.; Mori, E.. |
| Introdução e Objetivos: a prova biológica (PB) de inoculação intracerebral (IC) em camundongos com o vírus da raiva (RABV) apresenta sensibilidade diagnóstica superior ao teste de imunofluorescência direta (IFD). No entanto, o tempo necessário para o diagnóstico pela PB é maior que o consumido pela IFD, pois é dependente do período de incubação (PI) viral após inoculação IC. A determinação da duração desses períodos de observação (PO) em camundongos após inoculação IC com RABV foi estabelecida por observações empíricas. Com o intuito de estabelecer a duração do PO dos camundongos da linhagem Swiss inoculados IC com amostras clínicas (SNC, saliva e líquor) positivas para raiva, provenientes de diferentes espécies animais e humanos, oriundos do setor de... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2015 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/25665 |
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