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| Praça,Lílian Botelho; Batista,Andréa Cardoso; Martins,Érica Soares; Siqueira,Claúdia Brod; Dias,Daniel Gerheim de Souza; Gomes,Ana Cristina Menezes Mendes; Falcão,Rosana; Monnerat,Rose Gomes. |
| O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), Anthonomus grandis Boheman (Coleoptera: Curculionidae), Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae). Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Larva; Controle biológico; Proteína; Reação em cadeia da polimerase; Combate às pragas; Biopesticida. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000100002 |
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| Sihler,William; Souza,Marlinda Lobo de; Valicente,Fernando Hercos; Falcão,Rosana; Sanches,Marcio Martinello. |
| Abstract: The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Baculovirus; Biological control; Cell culture; Fall armyworm; In vitro production; SfMNPV. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100001 |
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| Monnerat,Rose Gomes; Dias,Daniel Gerhein Souza; Silva,Silvania Ferreira da; Martins,Erica Soares; Berry,Colin; Falcão,Rosana; Gomes,Ana Cristina Menezes Mendes; Praça,Lilian Botelho; Soares,Carlos Marcelo Silveira. |
| The objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culex quinquefasciatus; Aedes aegypti; Bioinsecticide. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000200001 |
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| Capdeville,Guy de; Souza Júnior,Manoel Teixeira; Santos,Jansen Rodrigo Pereira; Miranda,Simoni Paula; Caetano,Alexandre Rodrigues; Falcão,Rosana; Gomes,Ana Cristina Menezes Mendes. |
| The objective of this work was to investigate possible modes of action of the yeast Cryptococcus magnus in controlling anthracnose (Colletotrichum gloeosporioides) on post harvested papaya fruits. Scanning electron microscopy was used to analyze the effect of the yeast on inoculations done after harvest. Results showed that C. magnus is able to colonize wound surfaces much faster than the pathogen, outcompeting the later for space and probably for nutrients. In addition, C. magnus produces a flocculent matrix, which affects hyphae integrity. The competition for space and the production of substances that affect hyphae integrity are among the most important modes of action of this yeast. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya; Anthracnose; Antagonist yeast; Biological control; Competition. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007001100004 |
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| Medeiros,Patrícia Teles; Ferreira,Márcio do Nascimento; Martins,Érica Soares; Gomes,Ana Cristina Menezes Mendes; Falcão,Rosana; Dias,José Manoel Cabral de Souza; Monnerat,Rose Gomes. |
| O objetivo deste trabalho foi selecionar e caracterizar, no Banco de Germoplasma de Bacillus spp., da Empresa Brasileira de Pesquisa Agropecuária, as estirpes de Bacillus thuringiensis mais tóxicas à Plutella xylostella, por métodos morfológicos, bioquímicos e moleculares. Das 203 estirpes testadas, sete causaram 100% de mortalidade e foram semelhantes à estirpe padrão utilizada, B. thuringiensis subsp. kurstaki. Elas apresentaram proteínas de 130 kDa e 65 kDa, presença de genes cry1 e cry2 e cristais bipiramidais, cubóides e redondos. As estirpes selecionadas oferecem novas perspectivas de controle de P. xylostella. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cristais protéicos; Bactérias entomopatogênicas; Bioinseticidas. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001100014 |
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