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	Provedor de dados BABT (1) Autor Aiguang,Guo (1) Fangli,Wu (1) Jin,Liu (1) Weibo,Jin (1) Palavra-chave Endosperm-specific (1) Glutenin (1) Promoter (1) Transient expression (1) Triticum aestivum (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2009 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Cloning of high molecular weight gluten subunit promoter and study on its function in wheat BABT Weibo,Jin; Jin,Liu; Fangli,Wu; Aiguang,Guo. The aim of this work was to study the cloning and characterization of HMW-GS 1Dx2 promoter from Triticum aestivum. A 1050 bp partial promoter fragment including a putative TATA box and 5' encoding sequence of the gene was cloned by amplifying the upstream sequences using the nest-PCR with appropriate primers. The analysis of the promoter sequence against the PLACE (Plant cis-acting Regulatory DNA Elements) database showed the presence of certain putative endosperm-specific regulatory cis-elements in the sequence along with the TATA and CAAT boxes. The histochemical method detected the transient expressions of GUS in the seeds of wheat. The results showed that HMW-GS 1Dx2 promoter had the endosperm-specific transcription activity in the wheat seeds. Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Glutenin; Promoter; Endosperm-specific; Transient expression. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000200002 Registros recuperados: 1 Primeira ... 1 ... Última

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