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Sterilization of Exopolysaccharides Produced by Deep-Sea Bacteria: Impact on Their Stability and Degradation ArchiMer
Rederstorff, Emilie; Fatimi, Ahmed; Sinquin, Corinne; Ratiskol, Jacqueline; Merceron, Christophe; Vinatier, Claire; Weiss, Pierre; Colliec-jouault, Sylvia.
Polysaccharides are highly heat-sensitive macromolecules, so high temperature treatments are greatly destructive and cause considerable damage, such as a great decrease in both viscosity and molecular weight of the polymer. The technical feasibility of the production of exopolysaccharides by deep-sea bacteria Vibrio diabolicus and Alteromonas infernus was previously demonstrated using a bioproduct manufacturing process. The objective of this study was to determine which sterilization method, other than heat sterilization, was the most appropriate for these marine exopolysaccharides and was in accordance with bioprocess engineering requirements. Chemical sterilization using low-temperature ethylene oxide and a mixture of ionized gases (plasmas) was compared...
Tipo: Text Palavras-chave: Marine biotechnology; Polysaccharides; Sterilization procedures; Characterization; Molecular weight distribution; Rheology.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00027/13776/10913.pdf
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