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| Chatel, Annelle; Faucet-marquis, Virginie; Gourlay-france, Catherine; Pfohl-leszkowicz, Annie; Hubert, Francoise. |
| The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature.... |
| Tipo: Text |
Palavras-chave: Biomarker; Pollution; DNA adducts; Detoxification; Dreissena polymorpha; RT-PCR. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00195/30639/32450.pdf |
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| Chatel, Amelie; Faucet-marquis, Virginie; Pfohl-leszkowicz, Annie; Gourlay-france, Catherine; Vincent Hubert, Francoise. |
| Derived polycyclic aromatic hydrocarbons (PAHs) such as nitro-PAHs are present in the environment and are known to be much more toxic than PAHs compounds. However, very few studies have analysed their effects on the aquatic environment and none have investigated the freshwater environment. In the present study, we determined whether 1-nitropyrene (1-NP), a model of nitro-PAHs, can induce DNA adducts in gills and digestive glands of the freshwater mussel Dreissena polymorpha. Two concentrations of 1-NP (50 and 500 mu M) were tested. In addition, in order to understand the metabolic pathways involved in 1-NP genotoxicity, mRNA expression of genes implicated in biotransformation mechanisms was assessed by quantitative reverse transcription-PCR. Results showed... |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00287/39799/41532.pdf |
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| Chatel, Amelie; Faucet-marquis, Virginie; Perret, Marine; Gourlay-france, Catherine; Uher, Emmanuelle; Pfohl-leszkowicz, Annie; Vincent-hubert, Francoise. |
| The use of DNA adduct analysis has previously focused on the use of marine organisms for biomonitoring, whereas similar investigations in freshwater organisms are sparse. In that context, we have investigated the relevance of DNA adducts as biomarkers of genotoxicity in the freshwater mussels Dreissena polymorpha. The objective of the present study is to determine the stability of DNA adducts induced by benzo[a]pyrene (B[a]P) in zebra mussels. Mussels were exposed to dissolved B[a]P (10100 mu g/l) for 4 days. Afterwards, mussels were kept in clean water for 28 days and DNA adduct levels were subsequently measured in two different organs, the digestive glands and the gills, using the P-32-postlabelling technique. In parallel, the expression of genes... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00184/29566/27912.pdf |
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