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Picco,SJ; Seoane,A; Anchordoquy,JM; Anchordoquy,JP; Rosa,D; Fazzio,LE; Mattioli,GA; Furnus,CC. |
Zinc is an essential trace element. It has been known that a non adequate level of zinc can alter not only gene expression but also a variety of cellular functions with severe consequences on animal health. Little is known about the role of zinc on the reproductive performance of bovines, especially in oocyte maturation. The objective of the present study was to evaluate the impact of different zinc concentrations added to culture medium, on the DNA integrity of bovine cumulus cells during in vitro maturation of oocytes. Cumulus-oocyte coplexes obtained from an abattoir were cultured in IVM mediums containing 30 (G1), 70 (G2), 110 (G3) and 150 (G4) μg/dl. At the end of IVM, cumulus cells were processed for Comet Assay. Result obtained with the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zinc; Cumulus cells; Comet assay. |
Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332008000200004 |
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