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Molecular epidemiology and antimicrobial susceptibility of Enterococci recovered from Brazilian intensive care units BJID
Titze-de-Almeida,Ricardo; Rollo Filho,Maurício; Nogueira,Celeste A.; Rodrigues,Isabela P.; Eudes Filho,João; Nascimento,Rejane S. do; Ferreira II,Renato F.; Moraes,Lídia M. P.; Boelens,Hélène; Van Belkum,Alex; Felipe,Maria Sueli Soares.
We studied the antimicrobial resistance and the molecular epidemiology of 99 enterococcal surveillance isolates from two hospitals of Brasília, Brazil. Conventional biochemical tests were used to identify the enterococcal species and the disk diffusion method was used to determine their resistance profiles. Enterococcus faecalis (76%) and E. faecium (9%) were the most prevalent species. No enterococci showed the vanA or vanB vancomycin resistance phenotypes or genotypes. Only the intrinsically resistant species E. gallinarum (n=2) and E. casseliflavus (n=3) harbored the vancomycin-resistance genes vanC1 and vanC2/3, respectively. We found E. faecalis isolates with high-level resistance to gentamicin (22%) and streptomycin (8%) and both E. faecalis and E....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococci; Antimicrobial resistance; Genotyping.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000300002
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HIV-1 subtypes and mutations associated to antiretroviral drug resistance in human isolates from Central Brazil BJM
Cerqueira,Daniela Marreco; Ramalho,Eduardo Dias; Oliveira,Claudiner Pereira; Silva,Ruiter Roberto; Franchini,Miriam; Felipe,Maria Sueli Soares; Martins,Cláudia Renata Fernandes.
The detection of polymorphisms associated to HIV-1 drug-resistance and genetic subtypes is important for the control and treatment of HIV-1 disease. Drug pressure selects resistant variants that carry mutations in the viral reverse transcriptase (RT) and protease (PR) genes. For a contribution to the public health authorities in planning the availability of therapeutic treatment, we therefore described the genetic variability, the prevalence of mutations associated to drug resistance and the antiretroviral resistance profile in HIV-1 isolates from infected individuals in Central Brazil. Nineteen HIV-1 RNA samples from a Public Health Laboratory of the Federal District were reversely transcribed and cDNAs were amplified by nested PCR. One fragment of 297 bp...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HIV-1; Drug resistance; Antiretroviral drugs.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200003
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