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	Provedor de dados BABT (2) BJM (1) Autor Felix,Carlos Roberto (3) Guimarães,Valéria Monteze (1) Marco,Janice Lisboa De (1) Marco,Janice Lisboa de (1) Rezende,Sebastião Tavares de (1) Rodrigues,Marília de Castro (1) Valadares-Inglis,Maria Cléria (1) Mais... Palavra-chave 3-glucanase chitinase proteases (1) Alpha-galactosidase (1) Antagonistic interaction (1) Aspergillus fumigatus (1) Crinipellis perniciosa (1) Hydrolases (1) Raffinose oligosaccharides (1) Trichoderma (1) Trichoderma harzianum beta-1 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2007 (1) 2005 (1) 2003 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Purification and characterization of a beta-Glucanase produced by Trichoderma harzianum showing biocontrol potential BABT Marco,Janice Lisboa de; Felix,Carlos Roberto. A beta-1,3-glucanase was produced by Trichoderma harzianum in cultures containing chitin as the sole substrate. Two proteins showing beta-1,3-glucanase activity were purified to apparent homogeneity by hydrophobic chromatography. The molecular masses of these proteins were 29 and 36 kDa. The 36 kDa protein was further characterized. It was active on a broad pH range, and maximal activity was detected at pH 5.0. The optimum temperature of the 36 kDa beta-1,3-glucanase was 50ºC, but the purified enzyme was very sensitive to temperature. It lost about 60% or more of the activity after incubation for 30 min at 45, 50 and 60ºC. The apparent K M and Vmax for hydrolysis of laminarin at pH 5.0 and 37ºC, were 0.099 mg of reducing sugar/mL and 0.3 mg of reducing... Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma harzianum beta-1; 3-glucanase chitinase proteases. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100003 Purification and characterization of an alpha-galactosidase from Aspergillus fumigatus BABT Rezende,Sebastião Tavares de; Guimarães,Valéria Monteze; Rodrigues,Marília de Castro; Felix,Carlos Roberto. Aspergillus fumigatus secreted invertase (beta-fructofuranosidase) and alpha-galactosidase enzymatic activities able to hydrolyzing raffinose oligosaccharides (RO). alpha-Galactosidase was induced by galactose, melibiose and raffinose, but galactose was the most efficient inducer. It was purified by gel filtration and two ion exchange chromatographies and showed Mw of 54.7 kDa. The purified enzyme showed maximal activity against p-nitrophenyl-alpha-D-galactopyranoside (pNPGal) at pH 4.5-5.5 and 55 °C, and retained about 80% of the original activity after incubation for 90 minutes at 50ºC. The KM for pNPGal was 0.3 mM. Melibiose was hydrolyzed by the enzyme but raffinose was very poor substrate. Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus fumigatus; Alpha-galactosidase; Raffinose oligosaccharides. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200005 Production of hydrolytic enzymes by Trichoderma isolates with antagonistic activity against Crinipellis perniciosa, the causal agent of witches' broom of cocoa BJM Marco,Janice Lisboa De; Valadares-Inglis,Maria Cléria; Felix,Carlos Roberto. Two isolates of Trichoderma, which reduce the incidence of witches'broom disease caused in cocoa by Crinipellis perniciosa, were evaluated for their potential to produce hydrolases in liquid medium. Very low or no hydrolytic activity was produced in the absence of any substrate. The activities of chitinase, N-acetylglucosaminidase, beta-1,3-glucanase, total cellulase, endoglucanase, aryl- beta-glucosidase, beta-glucosidase, protease and amylase increased dramatically within 72-120 h of growth in the presence of specific substrates. Except for N-acetylglucosaminidase and beta-glucosidase Trichoderma harzianum isolate 1051 produced the largest amounts of hydrolases. The possible involvement of these enzymes in the antagonistic interaction between Trichoderma... Tipo: Info:eu-repo/semantics/article Palavras-chave: Trichoderma; Crinipellis perniciosa; Hydrolases; Antagonistic interaction. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100008 Registros recuperados: 3 Primeira ... 1 ... Última

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