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Genetic relationships between varieties of Stenocereus pruinosus (Cactaceae) in the states of Puebla and Oaxaca, Mexico, by means of AFLP markers Agrociencia
Yáñez-López,María de L.; Barbosa-Martínez,Claudia; Serrato-Díaz,Alejandra; Balderas-Morales,Atzin; Campos-Muñiz,Carolina; Fernández,Francisco J.; Armella-Villalpando,Miguel A..
Abstract: Pitaya de mayo, Stenocereus pruinosus (Otto) Buxbaum (Cactaceae), is a horticultural produce with great importance in the Mixteca region (Sowthwestern Puebla and Northeastern Oaxaca, México) and is gaining international attention for its sweet and fresh flavor; accordingly, many different cultivars or varieties were developed. In this study we used AFLP molecular markers with the objective of analyzing the genetic relationships between six varieties. Stenocereus stellatus (Pfeiffer) Ricobono variety was considered as outgroup, and the putative hybrid from these two species, San Gabriel, was grown in 12 plots of clonal reproduced plants at Tehuacán-Cuicatlán Region in Mexico. Results showed many polymorphisms, and similarity indexes fluctuated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Columnar cactus; Intraspecific variability; Pitaya; Stenocereus stellatus; Traditional handling.
Ano: 2016 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000500595
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Yeast HXK2 gene reverts glucose regulation mutation of penicillin biosynthesis in P. chrysogenum BJM
Pérez,Edmundo A.; Fernández,Francisco J.; Fierro,Francisco; Mejía,Armando; Marcos,Ana T.; Martín,Juan F.; Barrios-González,Javier.
The mutant Penicillium chrysogenum strain dogR5, derived from strain AS-P-78, does not respond to glucose regulation of penicillin biosynthesis and β-galactosidase, and is partially deficient in D-glucose phosphorilating activity. We have transformed strain dogR5 with the (hexokinase) hxk2 gene from Saccharomyces cerevisiae. Transformants recovered glucose control of penicillin biosynthesis in different degrees, and acquired a hexokinase (fructose phosphorylating) activity absent in strains AS- P-78 and dogR5. Interestingly, they also recovered glucose regulation of β-galactosidase. On the other hand, glucokinase activity was affected in different ways in the transformants; one of which showed a lower activity than the parental dogR5, but normal glucose...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucose regulation; Glucokinase; Penicillin; Penicillium chrysogenum.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300017
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