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	Provedor de dados BJMBR (1) Planta Daninha (1) R. Bras. Zootec. (1) Autor Fernandes,A.M. (3) Castilho,L.R. (1) Fontes,C.A.A. (1) Mariante,R.M. (1) Marinho,P.A.N. (1) Martins,C.E. (1) Paulsen,B.S. (1) Processi,E.F. (1) Rehen,S.K. (1) Rosolem,C.A. (1) Sant´Ana,N.F. (1) Sartore,R.C. (1) Siqueira,J.G. (1) Souza,E.F.C. (1) Souza-Schlick,G.D. (1) Mais... Palavra-chave Allelopathy (1) Human embryonic stem cells (1) Limousin (1) Microcarriers (1) Mombasa grass (1) No-tillage (1) Phaseolus vulgaris (1) Scale-up (1) Spinner bioreactors (1) Urochloa ruziziensis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2014 (1) 2011 (1) 2009 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system BJMBR Fernandes,A.M.; Marinho,P.A.N.; Sartore,R.C.; Paulsen,B.S.; Mariante,R.M.; Castilho,L.R.; Rehen,S.K.. Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up hES cell production. Maintenance of pluripotency in the microcarrier-based stirred system was shown by immunocytochemical and flow cytometry analyses for pluripotency-associated markers. The formation of cavitated embryoid bodies expressing markers of endoderm, ectoderm and mesoderm was further evidence of maintenance of differentiation capability. Cell yield per volume of medium spent was more than... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human embryonic stem cells; Scale-up; Microcarriers; Spinner bioreactors. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600007 Early growth of common bean cropped over ruzigrass residues Planta Daninha Souza,E.F.C.; Fernandes,A.M.; Souza-Schlick,G.D.; Rosolem,C.A.. Ruzigrass (Brachiaria ruziziensis, syn. Urochloa ruziziensis) is used as a cover crop in tropical regions because it has a high yield potential, is widely adapted and has a vigorous root system. However, it may affect early growth of the next crop due to allelopathy and competition for soil nitrate. A greenhouse experiment was conducted in glass-walled pots with soil to determine the effect of ruzigrass residues on the initial growth and mineral nutrition of common bean (Phaseolus vulgaris). Ruzigrass was grown in the pots for 50 days and chemically desiccated. Then, common bean was grown: without ruzigrass residues; with ruzigrass shoots placed on the soil surface; with ruzigrass roots left in the soil; and with ruzigrass shoots and roots left... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Urochloa ruziziensis; No-tillage; Allelopathy. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582014000400012 Energy partition for maintenance and weight gain in Nellore and crossbred steers finished under grazing R. Bras. Zootec. Sant´Ana,N.F.; Fontes,C.A.A.; Processi,E.F.; Siqueira,J.G.; Fernandes,A.M.; Martins,C.E.. The objective of this work was to estimate net energy (NEm) and metabolizable energy (MEm) requirements for maintenance and efficiency of use of metabolizable energy for maintanence (k m) and gain (k g) of grazing Nellore and crossbred steers. It was used 24 castrated steers, 12 Nellore breed (386 kg SBW) and 12 ½ Limousin-Nelore crossbred (397 kg SBW). The comparative slaughter method was used. In each genetic group, animals were grouped in three similar groups: reference; restrict feeding and ad libitum feeding. The reference group was slaughtered in the beginning of the experiment whereas the others were slaughtered at the end of it. During the 104 days of the experimental period, the group under restrict feeding had access to pastures for 3.5 hours... Tipo: Info:eu-repo/semantics/article Palavras-chave: Limousin; Mombasa grass. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000400029 Registros recuperados: 3 Primeira ... 1 ... Última

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