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Fernandes,F.F.. |
Investigaram-se os efeitos toxicológicos in vitro de três piretróides em diferentes concentrações, formulados para uso pecuário, domiciliar e em cães, sobre larvas de R. sanguineus, objetivando monitorar sua suscetibilidade e fomentar seu controle. Utilizaram-se 1.604 larvas em jejum, com 15 a 21 dias, obtidas por infestação artificial em cão, imersas nas soluções testadas, contidas em dispositivos constituídos por placa de Petri descartável, papel filtro e parafina, mantidas em incubadoras do tipo BOD e observadas ao estereoscópio por 24h. Observaram-se excitabilidade, movimentação repetitiva, diminuição da locomoção, desprendimento, paralisia, "knock-down" e proliferação cuticular de gases e líquidos. A mortalidade na 24ª hora foi de 86,9%, 100,0%,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carrapato; Rhipicephalus sanguineus; Piretróide. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000600012 |
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Fernandes,F.F.. |
In order to monitor the susceptibility of Boophilus microplus to acaricides and improve control measures, the effects of cypermethrin, deltamethrin and permethrin on larvae obtained in the city of Goiânia in the Brazilian state of Goiás were studied. Although these pyrethroids are already sold as acaricides, their cost-benefit efficiency has been questioned. Fasting 14-21 day-old larvae were immersed in solutions of the acaricides under test, maintained at 27±1° C, and relative humidity over 80%, and observed under the stereoscope within an apparatus originally designed for studying the non-parasitic phase of the tick life cycle. The observed toxicological effects were: excitability, repetitive motion, decreased motor ability, detachment, paralysis,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ixodidae; Boophilus microplus; Tick control; Pyrethroid; Acaricide resistance. |
Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000500004 |
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Peron,G.; Fernandes,F.F.; Landgraf,T.N.; Martinez,R.; Panunto-Castelo,A.. |
Paracoccidioides brasiliensis and P. lutzii are fungi that cause paracoccidioidomycosis (PCM), the most prevalent systemic mycosis in South America. For serological diagnosis, although 43-kDa glycoprotein (gp43) is regarded as highly specific for PCM, the occurrence of false negative reactions in sera from patients infected with P. lutzii suggests that preparation with only one antigen is not recommended. Heat shock proteins are feasible alternatives as a second antigen because they are often highly immunogenic. In this study, we evaluated the usefulness of recombinant 60-kDa heat shock protein from P. brasiliensis (rPbHsp60) for the serological diagnosis of PCM. Using western blotting assay, we observed that 77.3% of the sera from PCM patients were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paracoccidioidomycosis; Heat shock protein; Serodiagnosis; ELISA; Western blotting. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000400705 |
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