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Tumor growth reduction is regulated at the gene level in Walker 256 tumor-bearing rats supplemented with fish oil rich in EPA and DHA BJMBR
Borghetti,G.; Yamazaki,R.K.; Coelho,I.; Pequito,D.C.T.; Schiessel,D.L.; Kryczyk,M.; Mamus,R.; Naliwaiko,K.; Fernandes,L.C..
We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARγ), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (3×107 cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2-ΔΔCT method. FO significantly decreased tumor growth (W=13.18±1.58 vsWFO=5.40±0.88 g, P<0.05). FO supplementation also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cancer; Fish oil; Gene expression.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800696
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Modulation of peritoneal macrophage activity by the saturation state of the fatty acid moiety of phosphatidylcholine BJMBR
Grando,F.C.C.; Felício,C.A.; Twardowschy,A.; Paula,F.M.; Batista,V.G.; Fernandes,L.C.; Curi,R.; Nishiyama,A..
To determine the effects of saturated and unsaturated fatty acids in phosphatidylcholine (PC) on macrophage activity, peritoneal lavage cells were cultured in the presence of phosphatidylcholine rich in saturated or unsaturated fatty acids (sat PC and unsat PC, respectively), both used at concentrations of 32 and 64 µM. The treatment of peritoneal macrophages with 64 µM unsat PC increased the production of hydrogen peroxide by 48.3% compared to control (148.3 ± 16.3 vs 100.0 ± 1.8%, N = 15), and both doses of unsat PC increased adhesion capacity by nearly 50%. Moreover, 64 µM unsat PC decreased neutral red uptake by lysosomes by 32.5% compared to the untreated group (67.5 ± 6.8 vs 100.0 ± 5.5%, N = 15), while both 32 and 64 µM unsat PC decreased the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphatidylcholine; Lecithin; Macrophages; Fatty acids; Reactive oxygen species; Phagocytosis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000700003
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Time motion analysis of football (soccer) referees during official matches in relation to the type of fluid consumed BJMBR
Da Silva,A.I.; Fernandes,L.C.; Fernandez,R..
The aim of the present study was to determine the effect of volume and composition of fluid replacement on the physical performance of male football referees. Ten referees were evaluated during three official matches. In one match the participants were asked to consume mineral water ad libitum, and in the others they consumed a pre-determined volume of mineral water or a carbohydrate electrolyte solution (6.4% carbohydrate and 22 mM Na+) equivalent to 1% of their baseline body mass (half before the match and half during the interval). Total water loss, sweat rate and match physiological performance were measured. When rehydrated ad libitum (pre-match and at half time) participants lost 1.97 ± 0.18% of their pre-match body mass (2.14 ± 0.19 L). This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Football referee; Dehydration; Sweat loss; Performance; Fluid replacement.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800010
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Effect of chronic fish oil supplementation on renal function of normal and cachectic rats BJMBR
Fernandez,R.; Piechnik,J.; Fabris,R.; Malnic,G.; Fernandes,L.C..
In the present study we determined the effect of chronic diet supplementation with n-3 PUFA on renal function of healthy and cachectic subjects by providing fish oil (1 g/kg body weight) to female rats throughout pregnancy and lactation and then to their offspring post-weaning and examined its effect on renal function parameters during their adulthood. The animals were divided into four groups of 5-10 rats in each group: control, control supplemented with fish oil (P), cachectic Walker 256 tumor-bearing (W), and W supplemented with fish oil (WP). Food intake was significantly lower in the W group compared to control (12.66 ± 4.24 vs 25.30 ± 1.07 g/day). Treatment with fish oil significantly reversed this reduction (22.70 ± 2.94 g/day). Tumor growth rate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish oil supplementation; Renal function; Cachexia; Walker 256 tumor; Sodium excretion; Glomerular filtration rate.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000006
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