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Biodosimetry for dose assessment of partial-body exposure: a methodological improvement BABT
Fernandes,Thiago Salazar; Loyd,David C.; Amaral,Ademir.
This study has explored the possibility of combining culture times with extending the duration for which Colcemid is present in cell culture in order to obtain better dose estimations following partial-body exposures. Irradiated and unirradiated blood was mixed to simulate a partial-exposure. Dicentric frequencies and resultant dose estimations were compared from 48 and 72 h cultures with Colcemid added at the beginning, after 24 h or for the final 3 h. The frequencies of dicentrics in first division cells increased with the cell culture time, providing better dose estimations. Unwanted excessive contraction of chromosomes caused by prolonged contact with Colcemid was measured and ways to avoid this are discussed. It is suggested that the combination of a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodosimetry; Partial-body exposure; Dicentrics; Chromosome contraction.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700016
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Bioindicators in radiation protection BABT
Amaral,Ademir; Fernandes,Thiago Salazar; Cavalcanti,Mariana Brayner.
Biodosimetry is the evaluation of absorbed dose using bioindicators. Among chromosomal aberrations, scoring of dicentrics from peripheral human blood has been used as gold standard for biodosimetry, although in case of large scale incidents its use presents some drawbacks. Advances in technology have led to new investigations allowing or permitting the use of new methods which not only improve this "classical" biodosimetry but permits the design of other bioindicators making possible faster analyses, particularly in events where many persons may have been exposed. This report presents an overview of some recent studies developed by the "Grupo de Estudos em Radioproteção e Radioecologia - GERAR", Nuclear Energy Department of UFPE - Brazil, involving...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodosimetry; Physical Dosimetry; Radioprotection; Bioindicators; Protein expression.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700015
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