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	Provedor de dados BJMBR (6) Autor Fernandez,R. (6) Malnic,G. (3) Fernandes,L.C. (2) Tararthuch,A.L. (2) Biscaia,S.M.P. (1) Carraro-Lacroix,L.R. (1) Coutinho,A.G.G. (1) Da Silva,A.I. (1) Fabris,R. (1) Lessa,L.M.A. (1) Paes,M.R. (1) Piechnik,J. (1) Mais... Palavra-chave H+-ATPase (2) ATP (1) CAMP (1) Cachexia (1) Cell pH (1) Chloride channels (1) Cl- (1) Cl-/H+ Antiporter (1) Cytotoxicity (1) Dehydration (1) Energy expenditure (1) Fish oil supplementation (1) Fluid replacement (1) Football (soccer) (1) Football referee (1) Gentamicin (1) Glomerular filtration rate (1) Intracellular organelles (1) MDCK-C11 cells (1) Maximal oxygen uptake (VO2max) (1) Mais... Tipo do documento Info:eu-repo/semantics/article (6) Ano 2018 (1) 2016 (1) 2011 (1) 2009 (1) 2007 (1) 2004 (1) Mais... País Brazil (6) Idioma Inglês (6)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 6 Primeira ... 1 ... Última Effect of chronic fish oil supplementation on renal function of normal and cachectic rats BJMBR Fernandez,R.; Piechnik,J.; Fabris,R.; Malnic,G.; Fernandes,L.C.. In the present study we determined the effect of chronic diet supplementation with n-3 PUFA on renal function of healthy and cachectic subjects by providing fish oil (1 g/kg body weight) to female rats throughout pregnancy and lactation and then to their offspring post-weaning and examined its effect on renal function parameters during their adulthood. The animals were divided into four groups of 5-10 rats in each group: control, control supplemented with fish oil (P), cachectic Walker 256 tumor-bearing (W), and W supplemented with fish oil (WP). Food intake was significantly lower in the W group compared to control (12.66 ± 4.24 vs 25.30 ± 1.07 g/day). Treatment with fish oil significantly reversed this reduction (22.70 ± 2.94 g/day). Tumor growth rate... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish oil supplementation; Renal function; Cachexia; Walker 256 tumor; Sodium excretion; Glomerular filtration rate. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000006 Time motion analysis of football (soccer) referees during official matches in relation to the type of fluid consumed BJMBR Da Silva,A.I.; Fernandes,L.C.; Fernandez,R.. The aim of the present study was to determine the effect of volume and composition of fluid replacement on the physical performance of male football referees. Ten referees were evaluated during three official matches. In one match the participants were asked to consume mineral water ad libitum, and in the others they consumed a pre-determined volume of mineral water or a carbohydrate electrolyte solution (6.4% carbohydrate and 22 mM Na+) equivalent to 1% of their baseline body mass (half before the match and half during the interval). Total water loss, sweat rate and match physiological performance were measured. When rehydrated ad libitum (pre-match and at half time) participants lost 1.97 ± 0.18% of their pre-match body mass (2.14 ± 0.19 L). This... Tipo: Info:eu-repo/semantics/article Palavras-chave: Football referee; Dehydration; Sweat loss; Performance; Fluid replacement. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000800010 Cl- and regulation of pH by MDCK-C11 cells BJMBR Tararthuch,A.L.; Fernandez,R.; Malnic,G.. The interaction between H+ extrusion via H+-ATPase and Cl- conductance was studied in the C11 clone of MDCK cells, akin to the intercalated cells of the collecting duct. Cell pH (pHi) was measured by fluorescence microscopy using the fluorescein-derived probe BCECF-AM. Control recovery rate measured after a 20 mM NH4Cl acid pulse was 0.136 ± 0.008 pH units/min (dpHi/dt) in Na+ Ringer and 0.032 ± 0.003 in the absence of Na+ (0 Na+). With 0 Na+ plus the Cl- channel inhibitor NPPB (10 µM), recovery was reduced to 0.014 ± 0.001 dpHi/dt. 8-Br-cAMP, known to activate CFTR Cl- channels, increased dpHi/dt in 0 Na+ to 0.061 ± 0.009 and also in the presence of 46 nM concanamycin and 50 µM Schering 28080. Since it is thought that the Cl- dependence of H+-ATPase might... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell pH; CAMP; ATP; H+-ATPase; Valinomycin; Cl-. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500012 Physiological implications of the regulation of vacuolar H+-ATPase by chloride ions BJMBR Carraro-Lacroix,L.R.; Lessa,L.M.A.; Fernandez,R.; Malnic,G.. Vacuolar H+-ATPase is a large multi-subunit protein that mediates ATP-driven vectorial H+ transport across the membranes. It is widely distributed and present in virtually all eukaryotic cells in intracellular membranes or in the plasma membrane of specialized cells. In subcellular organelles, ATPase is responsible for the acidification of the vesicular interior, which requires an intraorganellar acidic pH to maintain optimal enzyme activity. Control of vacuolar H+-ATPase depends on the potential difference across the membrane in which the proton ATPase is inserted. Since the transport performed by H+-ATPase is electrogenic, translocation of H+-ions across the membranes by the pump creates a lumen-positive voltage in the absence of a neutralizing current,... Tipo: Info:eu-repo/semantics/article Palavras-chave: H+-ATPase; Chloride channels; Cl-/H+ Antiporter; Intracellular organelles; Plasma membranes. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200002 The aminoglycoside antibiotic gentamicin is able to alter metabolic activity and morphology of MDCK-C11 cells: a cell model of intercalated cells BJMBR Coutinho,A.G.G.; Biscaia,S.M.P.; Fernandez,R.; Tararthuch,A.L.. It is well known that the aminoglycoside antibiotic gentamicin is capable of causing damage to kidney cells. Given the known involvement of Ca2+ in the nephrotoxic action of gentamicin, the purpose of this study was to establish a relationship between the concentration of intracellular Ca2+ ([Ca2+]i) and cellular cytotoxicity using MDCK-C11 cells, a clone that has several properties that resemble those of intercalated cells of the distal nephron. Changes in [Ca2+]i was determined using fluorescence microscopy. Cell viability was evaluated by the neutral red method, and cell cytotoxicity by the MTT method. The [Ca2+]i gradually increased when cells were exposed to 0.1 mM gentamicin for 10, 20, and 30 min. The presence of extracellular Ca2+ was found to be... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gentamicin; [Ca2+]i; MDCK-C11 cells; Cytotoxicity. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001000610 Evaluation of energy expenditure in forward and backward movements performed by soccer referees BJMBR Paes,M.R.; Fernandez,R.. The aim of this study was to measure the energy expenditure for locomotor activities usually performed by soccer referees during a match (walking, jogging, and running) under laboratory conditions, and to compare forward with backward movements. The sample was composed by 10 male soccer referees, age 29±7.8 years, body mass 77.5±6.2 kg, stature 1.78±0.07 m and professional experience of 7.33±4.92 years. Referees were evaluated on two separate occasions. On the first day, maximal oxygen uptake (VO2max) was determined by a maximal treadmill test, and on the second day, the oxygen consumption was determined in different speeds of forward and backward movements. The mean VO2max was 41.20±3.60 mL·kg-1·min-1 and the mean heart rate achieved in the last stage of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Referee; Football (soccer); Energy expenditure; Maximal oxygen uptake (VO2max). Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000500702 Registros recuperados: 6 Primeira ... 1 ... Última

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