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| Silva,Fernanda R. da; Erdtmann,Bernardo; Dalpiaz,Tiago; Nunes,Emilene; Ferraz,Alexandre; Martins,Tales L.C.; Dias,Johny F.; Rosa,Darlan P. da; Porawskie,Marilene; Bona,Silvia; Silva,Juliana da. |
| Tobacco farmers are routinely exposed to complex mixtures of inorganic and organic chemicals present in tobacco leaves. In this study, we examined the genotoxicity of tobacco leaves in the snail Helix aspersa as a measure of the risk to human health. DNA damage was evaluated using the micronucleus test and the Comet assay and the concentration of cytochrome P450 enzymes was estimated. Two groups of snails were studied: one fed on tobacco leaves and one fed on lettuce (Lactuca sativa L) leaves (control group). All of the snails received leaves (tobacco and lettuce leaves were the only food provided) and water ad libitum. Hemolymph cells were collected after 0, 24, 48 and 72 h. The Comet assay and micronucleus test showed that exposure to tobacco leaves for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; Genotoxicity; Micronucleus test; Helix aspersa; Nicotiana tabacum; Tobacco leaves. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200018 |
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