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| Silva,SEL; Ferreira,HL; Carvalho,BA; Cardoso,TC. |
| Susceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Avibirnavirus; CER cells; Diagnosis; Double antibody sandwich ELISA; RT/PCR. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100009 |
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| Cardoso,TC; Oliveira,C; Da-Silva,SEL; Ferreira,HL; Pinto,AA. |
| Broilers were spray-vaccinated (n=150) with H120 serotype at one-day-old, challenged after 28 days with M41 IBV serotype and after bled at day 28, 34 and 46 after challenged. The respective sera were tested by the indirect ELISA (I-ELISA), sandwich ELISA (S-ELISA), liquid phase blocking ELISA (LPB-ELISA) and the standard serum neutralization test (SNT). For this purpose, a total of 300 sera samples, 150 from non vaccinated and 150 from vaccinated broilers were titrated by all the serological methods and the correlation coefficients were determined. The correlation values (r) between LPB-ELISA x SNT, S-ELISA x SNT, I-ELISA x SNT found werer = 0.98, r = 0.79, and r = 0.74, respectively. Nevertheless, the r between LPB-ELISA x S-ELISA, LPB-ELISA x I-ELISA and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ELISA; Post vaccinal response; Infectious bronchitis virus; Broilers. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2001000100010 |
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