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|  |  | Candeias,Marcela Leite; Alvarenga,Marco Antonio; Carmo,Márcio Teoro do; Ferreira,Heder Nunes; Maior,Mônica Russo Souto; Torres Filho,Rodolpho de Almeida; Rodrigues,André Luís Rios; Brandão,Felipe Zandonadi. |  | The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast... |  
| Tipo: Info:eu-repo/semantics/article | Palavras-chave: Ejaculated; Equine; Extender; Straw. |  
| Ano: 2012 | URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000900004 |  |  |