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| Dantas Junior,J.V.; Kimura,L.M.S.; Ferreira,M.S.R.; Fialho,A.M.; Almeida,M.M.S.; Grégio,C.R.V.; Romijn,P.C.; Leite,J.P.G.. |
| Este estudo teve por objetivo implantar um protocolo de amplificação genômica, precedida de transcrição reversa (RT-PCR) para o gene da nucleoproteína do vírus da raiva, para a utilização dessa metodologia em laboratórios onde são realizadas investigações para a detecção do vírus rábico. Foram utilizadas 50 amostras de tecido encefálico de animais (44 bovinos, 5 eqüinos e 1 quiróptero) oriundos do Estado do Rio de Janeiro, positivos por imunofluorescência direta e/ou prova biológica para o vírus rábico. A extração do RNA foi feita a partir da suspensão a 10% em PBS pH7,2 do tecido encefálico utilizando-se a metodologia de TRIzolTM (Life Technologies) e o protocolo de RT-PCR descrito por Heaton et al. (1997), incluindo algumas modificações. Dentre as 50... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Rabies virus; Reverse transcription-polymerase chain reaction; Diagnosis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300017 |
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| Teixeira,J.M.S.; Camara,G.N.N.L.; Pimentel,P.F.V.; Ferreira,M.N.R.; Ferreira,M.S.R.; Alfieri,A.A.; Gentsch,J.R.; Leite,J.P.G.. |
| Group C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Group C rotavirus; Diarrhea; HIV; RT-PCR; Probe hybridization. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100005 |
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| Xavier,M.P.T.P.; Oliveira,S.A.; Ferreira,M.S.R.; Victoria,M.; Miranda,V.; Silva,M.F.M.; Strina,A.; Barreto,M.L.; Miagostovicht,M.P.; Leite,J.P.G.. |
| Acute gastroenteritis caused by viruses is one of the leading causes of infantile morbidity. The aim of the present study was to investigate the presence of human caliciviruses of the genera norovirus and sapovirus in children up to 3 years of age with acute gastroenteritis from low-income communities in the city of Salvador, Brazil. This study is an extension of previous work carried out to establish the profile of the most prevalent enteric pathogens present in these communities. In this report, 139 fecal samples, collected from July 2001 to January 2002 were analyzed by RT-PCR and 13 (9%) were positive for human caliciviruses. By sequencing, seven isolates were characterized as norovirus genogroup GII and one as sapovirus genotype GII/1. Sequencing of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Calicivirus; Sapovirus; Astrovirus; Rotavirus A; Gastroenteritis; Genotypes. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000500007 |
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