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Evaluation of the Folin-Ciocalteu Method and Quantification of Total Tannins in Stem Barks and Pods from Libidibia ferrea (Mart. ex Tul) L. P. Queiroz BABT
Galvão,Marcos Aurelio Morais; Arruda,Alice Oliveira de; Bezerra,Isabelle Cristinne Ferraz; Ferreira,Magda Rhayanny Assunção; Soares,Luiz Alberto Lira.
ABSTRACT The stem bark and pods of “jucá” are widely used in Brazilian folk medicine and, despite their therapeutic relevancies, there are insufficient reports about their quality control. Both herbal drugs from the species are rich in tannins, which showed a lot of biological and economic interest. Thus, the purpose of this study was to evaluate the method for quantification of tannins in the stem bark and pods from L. ferrea. The performance of the method was tested against the influence of the most relevant parameters (reaction time; amount of drug; type and amount of precipitationagents; and, concentration of the reagents). The procedure was validated according to the literature. After optimization, the experimental conditions were established as: 30...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Libidibia ferrea; Quality control; Spectrophotometry; Tannins.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100431
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In vitro antifungal activity and phytochemical characterization of Eugenia uniflora, Libidibia ferrea and Psidium guajava BJPS
Ferreira,Magda Rhayanny Assunção; Santiago,Rosilene Rodrigues; Silva-Rocha,Walicyranison Plínio; Souza,Luanda Barbara Ferreira Canário de; Faria,Maria Graciela Icher; Mello,João Carlos Palazzo de; Langassner,Silvana Maria Zucolotto; Chaves,Guilherme Maranhão; Milan,Eveline Pipolo; Svidzinski,Terezinha Inez Estivalet; Soares,Luiz Alberto Lira.
The aim of this study was evaluate the in vitro antifungal activity of crude extracts from Eugenia uniflora, Libidibia ferrea and Psidium guajava. The extracts were obtained by turbo-extraction using water (AQ) or acetone-water (AC-W) (7:3, v/v) as solvents and lyophilized to obtain the crude extracts (CE). The CE were characterized by UV-Vis, TLC and HPLC. The activity of CEs was investigated against clinical isolates of Candida spp. and the Minimum Inhibitory Concentration (MIC), MIC50 and MIC90 were determinated. The analysis by TLC showed that all CEs presented polyphenols (flavonoids and tannins). The CEs from E. uniflora showed higher amount of polyphenols (30.35 ± 2.15%, AC-W) and the HPLC analysis revealed the tannins in all extracts. The CEs of E....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plants; Herbal extracts; Polyphenols; Candida isolates; Oral candidiasis.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100608
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