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Registros recuperados: 6
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Purification and characterization of a low molecular weight xylanase from solid-state cultures of Aspergillus fumigatus Fresenius Rev. Microbiol.
Silva,Claudio Henrique Cerri e; Puls,Jurgen; Sousa,Marcelo Valle de; Ferreira Filho,Edivaldo Ximenes.
A xylan-degrading enzyme (xylanase II) was purified to apparent homogeneity from solid-state cultures of Aspergillus fumigatus Fresenius. The molecular weight of xylanase II was found to be 19 and 8.5 kDa, as estimated by SDS-PAGE and gel filtration on FPLC, respectively. The purified enzyme was most active at 55 °C and pH 5.5. It was specific to xylan. The apparent Km and Vmax values on soluble and insoluble xylans from oat spelt and birchwood showed that xylanase II was most active on soluble birchwood xylan. Studies on hydrolysis products of various xylans and xylooligomers by xylanase II on HPLC showed that the enzyme released a range of products from xylobiose to xylohexaose, with a small amount of xylose from xylooligomers, and presented transferase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan; Xylanase; Aspergillus fumigatus.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200005
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Application of xylanases from Amazon Forest fungal species in bleaching of eucalyptus kraft pulps BABT
Medeiros,Roseli Garcia; Silva Jr.,Francides Gomes da; Báo,Sônia Nair; Hanada,Rogério; Ferreira Filho,Edivaldo Ximenes.
Crude xylanase preparations from Penicillium corylophilum, Aspergillus niger and Trichoderma longibrachiatum were used to treat Eucalyptus kraft pulp, prior to chlorine dioxide and alkaline bleaching sequences. The enzyme pretreatment improved brightness and delignification of non-delignified and oxygen-bleached samples of eucalyptus kraft pulp. Xylanase preparations from T. longibrachiatum and P. corylophilum were more effective to reduce pulp kappa number. A small reduction in viscosity was obtained when the oxygen-bleached pulp was treated with xylanase preparation from A. niger. For all enzyme samples, the best release of chromophoric material from the pulp was at 237 nm. The enzyme preparation from P. corylophilum was responsible for the highest...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xylan; Xylanase; Kraft pulp; Pulp bleaching.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200008
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A biotechnology perspective of fungal proteases BJM
Souza,Paula Monteiro de; Bittencourt,Mona Lisa de Assis; Caprara,Carolina Canielles; Freitas,Marcela de; Almeida,Renata Paula Coppini de; Silveira,Dâmaris; Fonseca,Yris Maria; Ferreira Filho,Edivaldo Ximenes; Pessoa Junior,Adalberto; Magalhães,Pérola Oliveira.
Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are essential for cell growth and differentiation. Proteolytic enzymes have potential application in a wide number of industrial processes such as food, laundry detergent and pharmaceutical. Proteases from microbial sources have dominated applications in industrial sectors. Fungal proteases are used for hydrolyzing protein and other components of soy beans and wheat in soy sauce production. Proteases can be produced in large quantities in a short time by established methods of fermentation. The parameters such as variation in C/N ratio, presence of some sugars, besides several other physical factors are important in the development of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteases; Enzyme production; Fungal protease; Industrial application.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200337
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The hydrolysis of agro-industrial residues by holocellulose-degrading enzymes BJM
Moreira,Leonora Rios de Souza; Ferreira,Gaspar Virgilio; Santos,Sheila Sousa Thurler; Ribeiro,Ana Paula Souza; Siqueira,Félix Gonçalves; Ferreira Filho,Edivaldo Ximenes.
Holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. Combinations of crude enzymatic extracts from different fungal species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger and Trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton residue to investigate the hydrolysis of holocellulose structures. A. terreus and A. oryzae were the best producers of FPase and xylanase activities. A combination of A. terreus and A. oryzae extracts in a 50% proportion provided optimal hydrolysis of dirty cotton residue and banana stem. For the hydrolysis of sugar cane bagasse, the best results were obtained with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural waste; Enzymatic mixtures; Hydrolysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200010
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Production and characterization of an enzyme complex from a new strain of Clostridium thermocellum with emphasis on its xylanase activity BJM
Vieira,Werner Bessa; Moreira,Leonora Rios de Souza; Monteiro Neto,Amadeu; Ferreira Filho,Edivaldo Ximenes.
A new bacterial strain (ISO II) was isolated from manure cow and identified as phylogenetically close to the thermophilic cellulolytic bacterium Clostridium thermocellum. The new strain produced extracellular xylanase, pectinase, mannanase and cellulase activities when grown in liquid culture medium containing banana stem as carbon source. The enzyme production profile after growth on banana stem showed that xylanase and cellulase activities were detected in different incubation periods. An enzyme complex containing xylanase, cellulase and mannanase activities was isolated from culture supernatant samples of strainISO II. The complex was partially purified by ultrafiltration and gel filtration chromatography on Sephacryl S-300. Zymogram analysis after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium thermocellum; Banana stem; Xylanase.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200009
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Production of xylan-degrading enzymes by a Trichoderma harzianum strain BJM
Cacais,André O.Guerreiro; Silveira,Fabiane Q. de Paula; Ferreira Filho,Edivaldo Ximenes.
Trichoderma harzianum strain 4 produced extracellular xylan-degrading enzymes, namely beta-xylanase, beta-xylosidase and alpha-arabinofuranosidase, when grown in liquid medium cultures containing oat spelt xylan as inducer. Cellulase activity was not detected. The pattern of xylan-degrading enzymes induction was influenced by the form of xylan present in the medium. They were detected in different incubation periods. Electrophoretic separation of the proteins from liquid culture filtrates by SDS-PAGE showed a variety of bands with high and low molecular weights.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Xylan; T. harzianum; Xylanolytic activity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200014
Registros recuperados: 6
Primeira ... 1 ... Última
 

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