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| Campos,Fabiana V.; Menezes,Thiago N.; Malacarne,Pedro F.; Costa,Fábio L. S.; Naumann,Gustavo B.; Gomes,Helena L.; Figueiredo,Suely G.. |
| Abstract The most poisonous fish species found along the Brazilian coast is the spotted scorpionfish Scorpaena plumieri. Though hardly ever life-threatening to humans, envenomation by S. plumieri can be quite hazardous, provoking extreme pain and imposing significant socioeconomic costs, as the victims may require days to weeks to recover from their injuries. In this review we will walk the reader through the biological features that distinguish this species as well as the current epidemiological knowledge related to the envenomation and its consequences. But above all, we will discuss the challenges involved in the biochemical characterization of the S. plumieri venom and its compounds, focusing then on the successful isolation and pharmacological... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpionfish; Scorpaena plumieri venom; Inflammatory response; Proteolytic activity; Cardiovascular activity; Sp-GP; Plumieribetin; C-type lectins; Sp-CTx. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100205 |
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| Costa,Fábio L. S.; Lima,Maria Elena De; Figueiredo,Suely G.; Ferreira,Rafaela S.; Prates,Núbia S.; Sakamoto,Tetsu; Salas,Carlos E.. |
| Abstract Background: Lethal factors are multifunctional oligomeric proteins found in the venomous apparatus of Scorpaeniformes fish. These toxins elicit not only an array of biological responses in vitro but also cardiovascular disorders and strong hemolytic, nociceptive and edematogenic activities in vivo. This work describes the cloning and molecular identification of two toxin subunits, denominated Sp-CTx-α and Sp-CTx-β, from scorpionfish venom ( Scorpaena plumieri ). Methods: The primary structures were deduced after cDNA amplification by PCR with primers from conserved sequences described in Scorpaeniformes toxins. Following DNA sequencing and bioinformatic analysis, the tridimensional structures of both subunits were modeled. Results: The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDNA; Lethal factor; Scorpaena plumieri; Scorpionfish; Venom gland. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100315 |
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| Costa,Fábio L. S.; Lima,Maria E. De; Pimenta,Adriano C.; Figueiredo,Suely G.; Kalapothakis,Evanguedes; Salas,Carlos E.. |
| Species of the family Scorpaenidae are responsible for accidents and sporadic casualties by the shore they inhabit. The species Scorpaena plumierifrom this family populate the Northeastern and Eastern coast of Brazil causing human envenomation characterized by local and systemic symptoms. In experimental animals the venom induces cardiotoxic, hypotensive, and airway respiratory effects. As first step to identify the venom components we isolated gland mRNA to produce a cDNA library from the fish gland. This report describes the partial sequencing of 356 gland transcripts from S. plumieri. BLAST analysis of transcripts showed that 30% were unknown sequences, 17% hypothetical proteins, 17% related to metabolic enzymes, 14% belonged to signal transducing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDNAs; EST; Glands; Lectin; Scorpionfish; Toxins. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000400871 |
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