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Molecular typing of Clostridium perfringens isolated from swine in slaughterhouses from São Paulo State, Brazil Ciência Rural
Ferreira,Thais Sebastiana Porfida; Moreno,Andrea Micke; Almeida,Renata Rodrigues de; Gomes,Cleise Ribeiro; Gobbi,Debora Dirani Sena de; Filsner,Pedro Henrique Nogueira de Lima; Moreno,Marina.
Clostridium perfringens is an anaerobic Gram-positive bacterium known as common pathogen for humans, for domestic and wildlife animals. Although infections caused by C. perfringens type C and A in swine are well studied, just a few reports describe the genetic relationship among strains in the epidemiological chain of swine clostridioses, as well as the presence of the microorganism in the slaughterhouses. The aim of the present study was to isolate C. perfringens from feces and carcasses from swine slaughterhouses, characterize the strains in relation to the presence of enterotoxin, alpha, beta, epsilon, iota and beta-2 toxins genes, using polymerase chain reaction (PCR) and comparing strains by means of Pulsed field gel electrophoresis (PFGE)....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; Swine; Slaughterhouse; PFGE; Carcass; Toxins.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000800020
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