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Behavioral meaningful opioidergic stimulation activates kappa receptor gene expression BJMBR
Teodorov,E.; Ferrari,M.F.R.; Fior-Chadi,D.R.; Camarini,R.; Felício,L.F..
The periaqueductal gray (PAG) has been reported to be a location for opioid regulation of pain and a potential site for behavioral selection in females. Opioid-mediated behavioral and physiological responses differ according to the activity of opioid receptor subtypes. The present study investigated the effects of the peripheral injection of the kappa-opioid receptor agonist U69593 into the dorsal subcutaneous region of animals on maternal behavior and on Oprk1 gene activity in the PAG of female rats. Female Wistar rats weighing 200-250 g at the beginning of the study were randomly divided into 2 groups for maternal behavior and gene expression experiments. On day 5, pups were removed at 7:00 am and placed in another home cage that was distant from their...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Real-time reverse-transcription polymerase chain reaction; Opioid receptor; Gene expression; Opioid sensitivity; Kappa opioid receptor.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012001000015
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Quantitative receptor radioautography in the study of receptor-receptor interactions in the nucleus tractus solitarii BJMBR
Fior-Chadi,D.R.; Fuxe,K..
The nucleus tractus solitarii (NTS) in the dorsomedial medulla comprises a wide range of neuropeptides and biogenic amines. Several of them are related to mechanisms of central blood pressure control. Angiotensin II (Ang II), neuropeptide Y (NPY) and noradrenaline (NA) are found in the NTS cells, as well as their receptors. Based on this observation we have evaluated the modulatory effect of these peptide receptors on <FONT FACE="Symbol">a</font>2-adrenoceptors in the NTS. Using quantitative receptor radioautography, we observed that NPY and Ang II receptors decreased the affinity of <FONT FACE="Symbol">a</font>2-adrenoceptors for their agonists in the NTS of the rat. Cardiovascular experiments agreed with the in vitro data....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Quantitative radioautography; Nucleus tractus solitarii; Alpha-2-adrenoceptors; Neuropeptide Y receptors; Angiotensin II receptors.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200006
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