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| Silva,Daniela Carla Medeiros; Moreira-Silva,Eduardo Augusto dos Santos; Gomes,Juliana de Assis Silva; Fonseca,Flávio Guimarães da; Correa-Oliveira,Rodrigo. |
| Vaccinia virus is responsible for a zoonosis that usually affects cattle and human beings in Brazil. The initial clinical signs of the infection are focal red skin areas, fever, and general symptoms similar to those of a cold. Then, pustules and ulcerated lesions surrounded by edema and erythema follow, as well as local lymphadenopathy that can last for weeks. Cure and healing of the lesions occur over several weeks, leaving a typical scar in the skin of people and animals affected. The infection definitive diagnosis is made through morphological characterization of the virus by use of electron microscopy, followed by PCR for specific viral genes. Since 1963, circulating orthopoxviruses in infectious outbreaks in several regions of Brazil have been... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Poxviridae infections; Virology; Outbreaks; Zoonoses; Vaccinia virus. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200003 |
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| Luppi,Marcela Miranda; Luiz,Ana Paula Moreira Franco; Coelho,Fabiana Magalhães; Ecco,Roselene; Fonseca,Flávio Guimarães da; Resende,Mauricio. |
| Abstract Thirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419 bp fragment of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Psittacid herpesvirus; PstI; RFLP; PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100217 |
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