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Nucleopolyhedrovirus: scanning electron microscopy technique Neotropical Entomology
Torquato,Ednéia F.B.; Miranda Neto,Marcílio H. de; Brancalhão,Rose M.C.; Franco,Valdeni S..
A simplified methodology was developed to study the geometric form of multiple Bombyx mori Nucleopolyhedrovirus by scanning electron microscopy. The virus belongs to Baculoviridae family and was isolated from the silkworm Bombyx mori (L.) (Lepidoptera: Bombycidae). The polyhedra of Nucleopolyhedrovirus were obtained from the filtrate, inoculum and hemolymph of the silkworm experimentally infected with nuclear polyhedra. This material was placed on stubs, where a copper tape was previously adhered. After dry at room temperature the virus was covered with carbon and gold. Scanning electron microscopy analysis reveled a well defined morphology for the polyhedra of multiple Bombyx mori Nucleopolyhedrovirus, making possible the mathematical study that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Baculovirus; Bombyx mori; BmMNPV; Polyhedra; Truncated octahedron.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2006000600011
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