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Screening for carbohydrate-binding proteins in extracts of Uruguayan plants 56
Plá,A.; Alonso,E.; Batista-Viera,F.; Franco Fraguas,L..
The presence of carbohydrate-binding proteins, namely lectins, ß-galactosidases and amylases, was determined in aqueous extracts of plants collected in Uruguay. Twenty-six extracts were prepared from 15 Uruguayan plants belonging to 12 Phanerogam families. Among them, 18 extracts caused hemagglutination (HAG) that was inhibited by mono- and disaccharides in 13 cases, indicating the presence of lectins. The other 8 extracts did not cause any HAG with the four systems used to detect HAG activity (rabbit and mouse red cells, trypsin-treated rabbit and mouse red cells). For the extracts prepared from Solanum commersonii, HAG activity and HAG inhibition were similar for those prepared from tubers, leaves and fruits, with the chitocompounds being responsible for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phanerogam families; Uruguayan plants; Asialofetuin-Sepharose; Carbohydrate-binding proteins; Lectins; SS-Galactosidases; Amylases.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700005
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