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Franke-Whittle, I.; Manici, L.; Insam , H.; Stes, B.. |
Background and aims: High-throughput 454 pyrosequencing was applied to investigate differences in bacterial and fungal communities between replant and closely situated control non-replant (fallow) soils. Methods The V1-V3 region of the bacterial 16S rRNA gene and the ITS1 region of fungi from the different soils were sequenced using 454 pyrosequencing (Titanium chemistry, and data were analysed using the MOTHUR pipeline. Results: The bacterial phyla Proteobacteria, Actinobacteria and Acidobacteria dominated in both fallow and replant apple orchard soils, and community composition at both phylum and genus level did not significantly differ according to NP-MANOVA. The fungal phyla Ascomycota, Zygomycota and Basidiomycota were dominant, and communities... |
Tipo: Journal paper |
Palavras-chave: Soil Fruit and berries Crop health; Quality; Protection. |
Ano: 2015 |
URL: http://orgprints.org/29109/1/10.1007_s11104-015-2562-x.pdf |
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