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| Freitas, A. P.; Peixoto, Z. M. P.; Silva, A. C. R.; Scheffer, K. C.; Chaves, L. B.; Caporale, G. M. M.. |
| The fluorescent anti-rabies conjugate (FARC) produced in Instituto Pasteur de São Paulo (IP-SP) is obtained by fluorescein isothiocyanate (FITC) conjugation with specific polyclonal antibodies purified and it recommended storage temperature of 4°C protected from light incidence. Given the fundamental importance of this reagent for rabies laboratorial diagnosis, the aim of this study was evaluate its stability in relation to physical factors that can affect the quality and maintenance of product characteristics. To do this, different aliquots of FARC batch IP-TOT1/11 submitted to temperatures of 37, 56, 65 and 80°C for 1hour or exposed to ambient light for 10, 20, 30, 45, 60, 90, 120, 150 minutes and 24 hours were used on direct immunefluorescence (DIF)... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3071 |
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| Freitas, A. P.; Silva, A. C. R.; Scheffer, K. C.; Pimenta, D. C.. |
| Rabies is an infectious disease caused by a virus belonging to the family Lissavirus that affects all mammals including humans. Rabies transmission occurs by virus-containing saliva , through biting or licking of an injured tissue. The virus replicates at the site of inoculation until it reaches the nerve endings. At the neuromuscular junctions, the virus binds specifically to the nicotinic acetylcholine receptor. On the other hand, alkaloids have yielded interesting results in terms of drug development. Since rabies virus infection is, at least, partially associated to the nicotinic acetylcholine receptor, the present study focus on the evaluation of molecules obtained from the skin secretion of Ameerega picta, in the process of penetration and infection... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2570 |
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