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	Provedor de dados BJM (3) BJID (1) Rev. Microbiol. (1) Autor Freitas,Ana Lúcia Peixoto de (5) Barth,Afonso Luís (3) Barth,Afonso Luis (2) Bender,Eduardo André (1) Lutz,Larissa (1) Machado,Denise Pires (1) Perez,Leandro Reus Rodrigues (1) Reiter,Keli Cristine (1) Soares,Fabiana da Silva Correa (1) Mais... Palavra-chave Pseudomonas aeruginosa (2) Resistance (2) Antibiotic resistance (1) Auxotrophy (1) Biofilm (1) Blood culture (1) Candidemia (1) Cystic fibrosis (1) ESBL (1) Enterococcus (1) HLAR (1) Imipenem (1) Klebsiella (1) Lysis-centrifugation system (1) Molecular typing (1) PFGE (1) Mais... Tipo do documento Info:eu-repo/semantics/article (5) Ano 2011 (1) 2009 (1) 2003 (1) 2002 (1) 1999 (1) País Brazil (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Role of the concentration process in the recovery of Candida albicans from blood Rev. Microbiol. Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luis. The lysis-centrifugation system (Isolator TM) is recognized as the standard method for recovery of Candida spp. from blood. In this study, the effect of the concentration process of this system was compared with conventional methods of blood culture using liquid and biphasic media. The tests were performed in vitro using Candida albicans in counts of 100, 10, 1, and 0.5 cells/ml of blood. Cultures onto chocolate agar were performed with the sediment obtained after centrifugation of IsolatorTM tube and with liquid and biphasic media after their incubation for 24 h at 35oC. Gram stain prepared from the conventional methods were also evaluated in the first 24 h. It was possible to detect Candida albicans in blood, regardless both the number of cells or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidemia; Blood culture; Lysis-centrifugation system. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000100011 Antibiotic resistance and molecular typing of Pseudomonas aeruginosa: focus on imipenem BJID Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luis. Susceptibility tests by disk diffusion and by E-test and molecular typing by macrorestriction analysis were performed to determine the relatedness of Pseudomonas aeruginosa isolates from three distinct hospitals. The resistance profile of 124 isolates to 8 antimicrobial agents was determined in three different hospitals, in Porto Alegre, Brazil. Frequencies of susceptibility ranged from 43.9% for carbenicillin to 87.7% for ceftazidime. Cross-resistance data of imipenem-resistant isolates indicated that most (70%) were also resistant to carbenicillin, although 30% remained susceptible to ceftazidime and cefepime. In general, susceptibility profiles were not able to determine relatedness among isolates of P. aeruginosa. On the other hand, molecular typing by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Antibiotic resistance; Imipenem. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702002000100001 Nutritional requirement among Pseudomonas aeruginosa isolates recovered from respiratory clinical specimens at a tertiary hospital from South of Brazil BJM Perez,Leandro Reus Rodrigues; Freitas,Ana Lúcia Peixoto de; Barth,Afonso Luís. We screened 349 isolates of P. aeruginosa from cystic fibrosis (CF+) and non-cystic fibrosis (CF-) patients for the auxotrophy. Fourteen (4.0%) were auxotrophic and among them only one was recovered from CF-patient showing that this characteristic is strongly associated with cystic fibrosis. In total, a requirement for 5 different compounds (or combination) was verified and, of these, methionine was the most common single amino acid required. Only one auxotrophic isolate was no able to produce biofilm in vitro. Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Auxotrophy; Cystic fibrosis; Biofilm. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300026 Extended-spectrum beta-lactamases in Klebsiella spp and Escherichia coli obtained in a Brazilian teaching hospital: detection, prevalence and molecular typing BJM Freitas,Ana Lúcia Peixoto de; Machado,Denise Pires; Soares,Fabiana da Silva Correa; Barth,Afonso Luís. His study was performed to compare the methods of detection and to estimate the prevalence of extended-spectrum beta-lactamases (ESBL) among Klebsiella spp and E.coli in a university hospital in southern Brazil. We also used a molecular typing method to evaluate the genetic correlation between isolates of ESBL K.pneumoniae. Production of ESBL was investigated in 95 clinical isolates of Klebsiella spp and Escherichia coli from Hospital de Clínicas de Porto Alegre, using Kirby-Bauer zone diameter (KB), double-disk diffusion (DD), breakpoint for ceftazidime (MIC CAZ), increased zone diameter with clavulanate (CAZ/CAC) and ratio of ceftazidime MIC/ceftazidime-clavulanate MIC (MIC CAZ/CAC). Molecular typing was performed by DNA macrorestriction analysis... Tipo: Info:eu-repo/semantics/article Palavras-chave: ESBL; Klebsiella; Resistance; Molecular typing. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400012 Identification, antimicrobial resistance and genotypic characterization of Enterococcus spp. isolated in Porto Alegre, Brazil BJM Bender,Eduardo André; Freitas,Ana Lúcia Peixoto de; Reiter,Keli Cristine; Lutz,Larissa; Barth,Afonso Luís. In the past two decades the members of the genus Enterococcus have emerged as important nosocomial pathogens worldwide. In the present study, we evaluated the antimicrobial resistance and genotypic characteristics of 203 Enterococcus spp. recovered from different clinical sources from two hospitals in Porto Alegre, Rio Grande do Sul, Brazil. The species were identified by conventional biochemical tests and by an automated system. The genetic diversity of E. faecalis presenting high-level aminoglycoside resistance (HLAR) was assessed by pulsed-field gel electrophoresis of chromosomal DNA after SmaI digestion. The E. faecalis was the most frequent specie (93.6%), followed by E. faecium (4.4%). The antimicrobial resistance profile was: 2.5% to ampicillin,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterococcus; Resistance; HLAR; PFGE. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300035 Registros recuperados: 5 Primeira ... 1 ... Última

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