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Embryonic stem-like cells derived from in vitro produced bovine blastocysts 52
Freitas,Erika Regina Leal de; Sanches,Bruno Valente; Gambarini,Maria Lúcia; Oliveira Filho,Benedito Dias; Guillo,Lidia Andreu.
The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF). Ten days after, primary outgrowths were mechanically dissected into several small clumps and transferred to a new MEF layer. Cells were further propagated and passaged by physical dissociation over a 60 days period. The pluripotency of the bovine ES-like cells was confirmed by RT-PCR of Oct-4 and STAT-3 gene markers. The colonies were weakly stained for alkaline phosphatase and the mesoderm and endoderm differentiation gene markers such as GATA-4 and Flk-1,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine blastocysts; ICM isolation; Embryonic stem cells; Gene markers.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300009
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