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In silico analysis of polymorphic microsatellites in penaeid shrimp and construction of a free-access database Genet. Mol. Biol.
Freitas,Patrícia Domingues de; Pinheiro,Allysson Pontes; Silva,Thiago Buosi; Galetti Junior,Pedro Manoel.
We performed an in silico analysis of all microsatellites so far described for penaeid shrimp and for which the polymorphic behavior has previously been analyzed. The objective of the study was to evaluate the structural characteristics of these microsatellites and identifying patterns which allow the characterization of the nature of these sequences in the penaeid genome. All data were compiled in a free-access database specially constructed for this study. Three hundred non-mononucleotide polymorphic microsatellite loci described for 12 shrimp species belonging to the family Penaeidae were analyzed and simple and compound microsatellites with di-, tri-, tetra-, penta- and hexanucleotide motifs were found. Dinucleotides and trinucleotides were the most...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crustacea; Microsatellite structure; Penaeidae; SSR.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600027
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Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae) Genet. Mol. Biol.
Gonçalves,Michelle Mantovani; Lemos,Manoel Victor Franco; Galetti Junior,Pedro Manoel; Freitas,Patrícia Domingues de; Furtado Neto,Manuel Antonio Andrade.
The Pacific white shrimp, Litopenaeus vannamei (Penaeidae), represents about 95% of all Brazilian shrimp production. The Brazilian L. vannamei foundation broodstock was made up of specimens collected from different American Pacific sites, but little information was collected on the genetic structure of the broodstock. We used the fluorescence amplified fragment length polymorphism (fAFLP) method to study the genetic diversity of L. vannamei broodstock lines 03CMF1 and 03CBF1 originally produced by breeder-shrimps imported mainly from Panama and Ecuador, although wild individuals from other localities may also have been used in producing these two lines. Our results showed a total of 93 polymorphic bands ranging from 50 to 500 bp, the mean Nei's genetic...
Tipo: Info:eu-repo/semantics/other Palavras-chave: FAFLP; Genetic diversity; Litopenaeus vannamei; Penaeidae; Shrimp.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200016
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Genetic variation in a closed line of the white shrimp Litopenaeus vannamei (Penaeidae) Genet. Mol. Biol.
Luvesuto,Eloize; Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The culture of the marine shrimp Litopenaeus vannamei has recently boosted the Brazilian shrimp industry. However, it is well known that selection methods based solely on phenotypic characteristics, a reduced number of breeders and the practice of inbreeding may promote a significant raise in the genetic similarity of the captive populations, leading to greater disease susceptibility and impairing both the growth and final size of the shrimps. We used four microsatellite loci to investigate genetic variation in three generations (F5, F6 and F7) of a closed and reared L. vannamei lineage. Although an accentuated heterozygosis deficit was detected, we also observed that the captive propagation of this lineage did not lead to a significant loss of genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broodstocks; Inbreeding; Penaeids; Selection.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600021
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PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei Genet. Mol. Biol.
Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Genetic diversity; Shrimp; VNTR; PCR.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012
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Polymorphism in Litopenaeus vannamei genes and cross-species amplification in other shrimp species PAB
Santos,Camilla Alves; Farias,Flavio; Teixeira,Ana Karina; Rocha,João Luís; Guerrelhas,Ana Carolina; Freitas,Patrícia Domingues de.
Abstract: The objective of this work was to assess polymorphisms within Litopenaeus vannamei candidate genes related to growth performance, and a possible association between weight genotypes and phenotypes in two shrimp closed lines. Three EST-SSR (serine-arginine, 60S ribosomal, and troponin) and one EST-SNP (crustacyanin) were selected and evaluated in L. vannamei and seven other marine shrimp species, besides two freshwater species. Genotypes had no effect on total body weight. Although no association was observed in the sampled population, these markers showed amplification for most of the evaluated species and, therefore, may be useful in further genetic studies on shrimp species.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candidate genes; Crustacyanin; Microsatellite; SNP; Weight-association; 60S ribosomal.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100121
Registros recuperados: 5
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