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Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods Biocell
Frescura,Viviane Dal-Souto; Laughinghouse IV,Haywood Dail; Dorow,Thais Scotti do Canto-; Tedesco,Solange Bosio.
Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of P. paniculata using the Alexander's stain, which proved the most adequate method to estimate pollen...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Pollen grains; Barba-de-bode; Acetic orcein; Acetic carmine; Alexander's stain.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452012000300007
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Genotoxic and chromatographic analyses of aqueous extracts of Peltodon longipes Kunth ex Benth. (hortelã-do-campo) BJPS
Kuhn,Andrielle Wouters; Tedesco,Marília; Boligon,Aline Augusti; Frescura,Viviane Dal-Souto; Athayde,Margareth Linde; Tedesco,Solange Bosio.
Peltodon longipes is used as a stimulant and emmenagogue. The objective of this study was to perform genotoxic and chromatographic analyses of the extracts of two samples of P. longipes, collected from the cities of Santa Maria and Tupanciretã, RS, Brazil. The Allium cepa assay was used to analyze genotoxicity while high-performance liquid chromatography was employed to determine phenolic compounds. The genotoxicity experiment consisted of nine groups each comprising four A. cepa bulbs. Bulb roots were developed in distilled water and then transferred for the treatments, for 24 hours, and the negative control remained in water. The treatments were: aqueous extracts at concentrations of 5 and 15 g L-1 for each sample, plus four groups treated with 1%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: P. longipes/antiproliferative effect; P. longipes/genotoxic potential; P. longipes/chromatographic analyses; Allium cepa test/genotoxicity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300533
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