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	Provedor de dados Electron. J. Biotechnol. (2) Autor Fu,Junjiang (2) Khan,Asaduzzaman (2) Cheng,Jingliang (1) Fu,Shelly (1) Long,Yan (1) Luo,Peiyi (1) Mei,Zhiqiang (1) Tania,Mousumi (1) Wei,Chunli (1) Zhang,Chun (1) Zhu,Ye (1) Mais... Palavra-chave Genetic authentication (2) Random amplified polymorphic DNA (2) Angelica sinensis (1) Inter-simple sequence repeat (1) Molecular cloning (1) Sequence-characterized amplified region marker (1) Substitutes (1) Mais... Tipo do documento Journal article (2) Ano 2015 (2) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China Electron. J. Biotechnol. Mei,Zhiqiang; Zhang,Chun; Khan,Asaduzzaman; Zhu,Ye; Tania,Mousumi; Luo,Peiyi; Fu,Junjiang. Background Angelica sinensis is a well-known traditional Chinese medicinal plant. We aimed to assess the genetic diversity and relationships in A. sinensis cultivars collected from different locations of China and also some other Angelica species. Results We employed an improved random amplified polymorphic DNA (RAPD) technique for the amplification of DNA materials from ten Angelica cultivars, and the results were verified by inter-simple sequence repeat (ISSR) analysis. Twenty six RAPD primers were used for RAPD, and the amplified bands were found highly polymorphic (96%). Each primer amplified 8-14 bands with an average of 10.25. The cluster dendrogram showed that the similarity coefficients ranged from 0.41 to 0.92. The similarity coefficients were... Tipo: Journal article Palavras-chave: Angelica sinensis; Genetic authentication; Inter-simple sequence repeat; Random amplified polymorphic DNA; Substitutes. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200006 Development and significance of RAPD-SCAR markers for the identification of Litchi chinensis Sonn: by improved RAPD amplification and molecular cloning Electron. J. Biotechnol. Cheng,Jingliang; Long,Yan; Khan,Asaduzzaman; Wei,Chunli; Fu,Shelly; Fu,Junjiang. Background Analysis of genetic diversity is important for the authentication of a species. Litchi (Litchi chinensis Sonn.) is a subtropical evergreen tree. Recently, L. chinensis has been characterized by an improved random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analysis. The goal of this study was to develop sequence-characterized amplified region (SCAR) markers from the improved RAPD fragments for the genetic analysis of L. chinensis. Results The improved RAPD fragments from L. chinensis were cloned, sequenced and converted into stable SCAR markers. Sequencing of three cloned RAPD fragments revealed that the clone L7-16 consisted of 222 nucleotides (GenBank accession number KM235222), clone L9-6 consisted of 648... Tipo: Journal article Palavras-chave: Genetic authentication; Molecular cloning; Random amplified polymorphic DNA; Sequence-characterized amplified region marker. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100007 Registros recuperados: 2 Primeira ... 1 ... Última

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