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| Registros recuperados: 14 | |
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| Fukuda, Kenji; Shi, Tala; Nagami, Kentaro; Leo, Fiame; Nakamura, Tadashi; Yasuda, Kumi; Senda, Akitsugu; Motoshima, Hidemasa; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| A thermophilic lactic acid bacterium, Lactobacillus fermentum TDS030603, produced about 100 mg/L of EPS in purified form when grew in de Man-Rogosa-Sharpe (MRS) broth. The 1% (w/v) solution of the purified EPS was highly viscous, exhibiting an apparent 22 viscosity (ηapp) of 0.88 Pa s at a shear rate of 10/s. To investigate the impact of carbohydrate source on the production yield and chemical structure of EPS and the viscosity of EPS solution, a chemically defined medium (CDM) has been developed. Results of TLC, HPLC, 25 and 1H NMR spectroscopy indicated that the chemical structures of EPS released in MRS and in the CDM supplemented with glucose, galactose, lactose or sucrose were very similar. All the 1% solutions of EPSs released in CDMs were highly... |
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Palavras-chave: Chemical structure; Chemically defined medium; Exopolysaccharide; Lactic acid bacteria; Viscosity. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2694 |
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| Hirayama, Kentaro; Taufik, Epi; Kikuchi, Megumi; Nakamura, Tadashi; Fukuda, Kenji; Saito, Tadao; Newgrain, Keith; Green, Brian; Messer, Michael; Urashima, Tadasu. |
| Previous structural characterizations of marsupial milk oligosaccharides have been performed in the tammar wallaby, red kangaroo, koala, common brushtail possum and the eastern quoll. To clarify the homology and heterogeneity of milk oligosaccharides among marsupial species, which could provide information on their evolution, the oligosaccharides of wombat milk carbohydrate were characterized in this study. Neutral and acidic oligosaccharides were isolated from the carbohydrate fractions of two samples of milk of the common wombat and characterized by H-1-nuclear magnetic resonance spectroscopy. The structures of six neutral saccharides were found to be Gal(1-4)Glc (lactose), Gal(1-3)Gal(1-4)Glc (3'-galactosyllactose), Gal(1-3)Gal(1-3)Gal(1-4)Glc... |
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Palavras-chave: Common wombat; Marsupial; Milk oligosaccharide; Vombatus ursinus. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4471 |
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| Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6... |
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Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183 |
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| 福田, 健二; 浦島, 匡; Fukuda, Kenji; Urashima, Tadasu. |
| Lactic acid bacteria (LAB) are commonly used in food industries，especially for the production of fermented dairy products. During fermentation process，some dairy LAB produce exopolysaccharide(EPS)，providing good mouthfeel properties to the products by modifying their textures. In general， EPS is divided into two groups based on their monosaccharide composition; homo-EPS and hetero-EPS. It is believed that the major physiological function of EPS is the first line of biological defense against phagocytosis，phage attack， antibiotics，toxic metal ions，and physical stresses such as desiccation and osmotic stress. Besides，EPS from dairy LAB exhibits bioactive characteristics，e.g.，anti-microbial，anti-tumoral，hypotensive，prebiotic，immuno-stimulative，and... |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3080 |
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| Goto, Kohta; Fukuda, Kenji; Senda, Akitsugu; Saito, Tadao; Kimura, Kazumasa; Glander, Kenneth E.; Hinde, Katie; Dittus, Wolfgang; Milligan, Lauren A.; Power, Michael L.; Oftedal, Olav T.; Urashima, Tadasu; 浦島, 匡; 福田, 健二. |
| Human and great ape milks contain a diverse array of milk oligosaccharides, but little is known about the milk oligosaccharides of other primates, and how they differ among taxa. Neutral and acidic oligosaccharides were isolated from the milk of three species of Old World or catarrhine monkeys (Cercopithecidae: rhesus macaque (Macaca mulatta), toque macaque (Macaca sinica) and Hamadryas baboon (Papio hamadryas)) and three of New World or platyrrhine monkeys (Cebidae: tufted capuchin (Cebus apella)and Bolivian squirrel monkey (Saimiri boliviensis); Atelidae: mantled howler (Alouatta palliata)). The milks of these species contained 6-8% total sugar, most of which was lactose: the estimated ratio of oligosaccharides to lactose in Old World monkeys (1:4 to... |
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Palavras-chave: Old world monkey; New world monkey; Milk oligosaccharide; Rhesus macaque; Toque macaque; Baboon; Capuchin; Mantled howler; Squirrel monkey; N-glycolylneuraminic acid. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3144 |
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| Urashima, Tadasu; Kobayashi, Mami; Asakuma, Sadaki; Uemura, Yusuke; Arai, Ikichi; Fukuda, Kenji; Saito, Tadao; Mogoe, Toshihiro; Ishikawa, Hajime; Fukui, Yutaka. |
| Samples of milk from a Bryde's whale and a Sei whale contained 2.7 g/100 mL and 1.7 g/100 mL of hexose, respectively. Both contained lactose as the dominant saccharide along with small amounts of Neu5Ac(alpha 2-3)Gal(beta 1-4)Glc(3'-N-acetylneuraminyllactose), Neu5Ac(alpha 2-6)Gal(beta 1-4) Glc(6-N- acetylneuraminyllactose) and Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc (LST c). The dominance of lactose in the carbohydrate of these milks is similar to that of Minke whale milk and bottlenose dolphin colostrum, but the oligosaccharide patterns are different from those of these two species, illustrating the heterogeneity of milk oligosaccharides among the Cetacea. (c) 2006 Elsevier Inc. All rights reserved. |
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Palavras-chave: Bryde's whale; Sei whale; Milk oligosaccharides; Balaenoptera edeni; Balaenoptera borealis lesson; Cetacea. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1659 |
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| Urashima, Tadasu; Yamamoto, Tomoko; Hirayama, Kentaro; Fukuda, Kenji; Nakamura, Tadashi; Saito, Tadao; Newgrain, Keith; Merchant, Jim; Green, Brian; Messer, Michael. |
| Milk oligosaccharides were separated from the carbohydrate fraction of milk of the tiger quoll a species of marsupial that is closely related to the eastern quoll, Dasyurus viverrinus. They were characterized by H-1 - nuclear magnetic resonance spectroscopy and matrix - assisted laser desorption/ionization time-of-flight mass spectrometry. The following oligosaccharides were identified; Gal(beta 1-3) Gal(beta 1-4) Glc, Gal(beta 1-3) Gal(beta 1-3) Gal(beta 1-4) Glc, Gal(beta 1-3)[Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-4) Glc, Gal(beta 1-3) Gal(beta 1-3)[Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-4) Glc, Gal(beta 1-3)[Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-3) Gal(beta 1-4) Glc, Gal(beta 1-3)[Gal(beta 1-3) Gal(beta 1-4) GlcNAc(beta 1-6)] Gal(beta 1-4)... |
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Palavras-chave: Tiger quoll; Milk oligosaccharides; Marsupials. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4528 |
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| Asakuma, Sasaki; Yokoyama, Tomoko; Kimura, Kazumasa; Watanabe, Yoko; Nakamura, Tadashi; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| It is believed that human milk oligosaccharides (HMO) act as receptor analogues that inhibit the attachment of pathogenic microorganisms to the infant colon, but the direct involvement of HMO in anti infection has not as yet been demonstrated. The present study was conducted to clarify whether HMOs as well as commercially available galacto oligosaccharides (GO), from which lactose has been removed, affect the expression of toll-like receptor (TLR) 2 and 4, and of MD2 mRNA in the HT-29, human colonic cell line. HT-29 cells were treated with neutral human milk oligosaccharides (nHMOs), which had been separated from the acidic oligosaccharide fraction and also from lactose, as well as with GOs. HT-29 cells were treated with specific oligosaccharides of major... |
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Palavras-chave: Human milk oligosaccharides; Galacto-oligosaccharides; Toll-like receptor; Intestinal cell; HT-29 cells. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4184 |
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| Registros recuperados: 14 | |
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