|
|
|
|
|
| Almeida,C.N.; Furian,T.Q.; Borges,K.A.; Perdoncini,G.; Mauel,M.J.; Rocha,S.L.S.; Nascimento,V.P.; Salle,C.T.P.; Moraes,H.L.S.. |
| ABSTRACT Fowl Cholera (FC) is a disease caused by Pasteurella multocida. The severity of this disease is partly caused by virulence factors. Genes encoding fimbriae, capsule, sialidases and proteins for iron metabolism may be related to P. multocida’s ability to infect the host. Besides to examining DNA for the presence of virulence genes, DNA is essential for the diagnostic and FTA cards are an alternative for genetic material transport. The study aims to evaluate the viability of P. multocida DNA transport using the cards and to detect 14 virulence genes in 27 strains isolated from FC cases in the United States by multiplex-PCR. No growth was observed in any of the FTA cards, which was essential to assess the security. Furthermore, DNA detection was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fowl cholera; FTA cards; Virulence genes. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601855 |
| |
|
|
| Lima,D.A.; Furian,T.Q.; Pillati,R.M.; Silva,G.L.; Morgam,R.B.; Borges,K.A.; Fortes,F.B.B.; Moraes,H.L.S.; Brito,B.G.; Brito,K.C.T.; Salle,C.T.P.. |
| Salmonella Enteritidis and Salmonella Typhimurium are responsible for causing huge economic loses in aviculture, as they lead young broiler chicks to develop clinical disease and thus increase mortality. Salmonella's pathogenicity is considered complex and multifactorial, demanding more studies that could elucidate the interaction between host and pathogen. The present study aims to evaluate the virulence of 130S. Enteritidis isolates and 70S. Typhimurium inoculated in one-day-old chicks through the establishment of a pathogenicity index. For each strain, 10 commercial chicks from the Cobb lineage were used. Then, 200µL of a solution containing 2x108 CFU of S. Enteritidis or S. Typhimurium were inoculated in the birds by intraperitoneal via. Mortality and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Birds; Salmonelosis; Virulence. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200257 |
| |
|
|
|
