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| GARRICK,MICHAEL D; SINGLETON,STEVEN T; VARGAS,FARIDA; KUO,H-C; ZHAO,LIN; KNÖPFEL,MARTIN; DAVIDSON,TODD; COSTA,MAX; PARADKAR,PRASAD; ROTH,JEROME A; GARRICK,LAURA M. |
| DMT1 _ Divalent Metal (Ion) Transporter 1 or SLC11A2/DCT1/Nramp2 _ transports Fe2+ into the duodenum and out of the endosome during the transferrin cycle. DMT1 also is important in non-transferrin bound iron uptake. It plays similar roles in Mn2+ trafficking. Voltage clamping showed that six other metals evoked currents, but it is unclear if these metals are substrates for DMT1. This report summarizes progress on which metals DMT1 transports, focusing on results from the authors' labs. We recently cloned 1A/+IRE and 2/-IRE DMT1 isoforms to generate HEK293 cell lines that express them in a tetracycline-inducible fashion, then compared induced expression to uninduced expression and to endogenous DMT1 expression. Induced expression increases ~50x over... |
| Tipo: Journal article |
Palavras-chave: Metal ion transport; DCT1; Nramp2 or SLC11A2; Expression assay; Competition between metal ions. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100009 |
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