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| Jouault, Albane; Gobet, Angelique; Simon, Marjolaine; Portier, Emilie; Perennou, Morgan; Corre, Erwan; Gaillard, Fanny; Vallenet, David; Michel, Gurvan; Fleury, Yannick; Bazire, Alexis; Dufour, Alain. |
| The aim was to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another non-active Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein including a predicted 20-residue... |
| Tipo: Text |
Palavras-chave: Antibiofilm protein; Pseudoalteromonas; Pseudomonas aeruginosa; Biofilm. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75517/76405.pdf |
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| Le Lann, Klervi; Surget, Gwladys; Couteau, Celine; Coiffard, Laurence; Cerantola, Stephane; Gaillard, Fanny; Larnicol, Maud; Zubia, Mayalen; Guerard, Fabienne; Poupart, Nathalie; Stiger-pouvreau, Valerie. |
| The present study focused on a brown macroalga (Halidrys siliquosa), with a particular emphasis on polyphenols and their associated biological activities. Two fractions were obtained by liquid/liquid purification from a crude hydroethanolic extract: (i) an ethyl acetate fraction and (ii) an aqueous fraction. Total phenolic contents and antioxidant activities of extract and both fractions were assessed by in vitro tests (Folin-Ciocalteu test, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power assay, superoxide anion scavenging assay, and beta-carotene-linoleic acid system). For the most active fraction, i.e., the ethyl acetate fraction, the oxygen radical absorbance capacity (ORAC) value, antibacterial activities, and sunscreen... |
| Tipo: Text |
Palavras-chave: Antioxidant; Bactericidal; Phenolic compounds; Phaeophyceae; Seaweed; ORAC value; Sunscreen potential. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00366/47682/47970.pdf |
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