SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Campana,Eloiza Helena; Xavier,Danilo Elias; Petrolini,Fernanda Villas-Boas; Cordeiro-Moura,Jhonatha Rodrigo; Araujo,Maria Rita Elmor de; Gales,Ana Cristina.

Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed...

Misidentiﬁcation of pan drug-resistant Klebsiella pneumoniae clinical isolates as a metallo-β-lactamase producers by the EDTA/DDST test

Palavras-chave: Gram-negative bacilli; Carbapenem resistance; OprD porin.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100057

Cassu-Corsi,Dandara; Martins,Willames M.B.S.; Scheffer,Mara Cristina; Cayô,Rodrigo; Gales,Ana Cristina.

Carbapenem-resistant Pseudomonas aeruginosa: clonal spread in southern Brazil and in the state of Goiás

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000100102

Scheffer,Mara Cristina; Gales,Ana Cristina; Barth,Afonso Luis; Carmo Filho,José Rodrigues do; Dalla-Costa,Libera Maria.

This study evaluated the clonal spread of carbapenem-resistant P. aeruginosa producing SPM-1 type metallo-Î²-lactamase (MBL), at the university hospital of Florianópolis, Santa Catarina, Brazil, compared to an epidemic clone previously reported, as well as strains collected in other three Brazilian states. Among the isolates, 17 (62%) were clonal and highly related to strains from other regions of Brazil. Six clonal strains harbored the blaSPM-1 gene. The finding of a unique SPM-1 producer clone suggests that its dissemination has contributed to the high resistance to carbapenems in Brazilian hospitals

Clinical and microbiological characterization of KPC-producing Klebsiella pneumoniae infections in Brazil

Palavras-chave: P. aeruginosa; Carbapenem resistance; Metallo- Β-lactamase.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500014

Beirão,Elisa Maria; Furtado,Juvencio Jose Duailibe; Girardello,Raquel; Ferreira Filho,Heli; Gales,Ana Cristina.

In 2008 isolates of KPC-producing Klebsiella pneumoniae (KPC-KPN) were detected for the first time at Hospital Heliópolis, São Paulo, Brazil. The aim of this study was to characterize the clinical and microbiological outcomes of infections caused by KPC-KPN. A historical cohort of patients from whom KPC-KPN strains were isolated was performed. Isolates were identified as resistant to ertapenem by automated broth microdilution system and screened as carbapenemase producers by the modified Hodge test. The beta-lactamase resistance gene blaKPC was detected by PCR. The genetic relatedness of isolates was determined by PFGE. The study provides early clinical experience in treating KPC-KPN infections in a Brazilian tertiary center.

Quinolone-resistant Escherichia coli

Palavras-chave: Klebsiella pneumonia; KPC; Hospital infection; Carbapenemase.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100013

Ito,Carmen Antonia Sanches; Gales,Ana Cristina; Tognim,Maria Cristina B.; Munerato,Patrícia; Dalla Costa,Libera Maria.

Quinolones (nalidixic acid - NAL, norfloxacin - NOR, ciprofloxacin - CIP and gatifloxacin - GAT) were tested against Escherichia coli isolated from urine (385 patient samples) by disk diffusion (DD) and agar dilution (AD) methods. Fifty-three samples (13.8%) were classified as resistant to at least one of the quinolones tested. CIP and NOR susceptibilities were the same (91.4%) and they were similar to GAT (92.7%). Susceptibility to NAL, detected by the disk diffusion method, was used to predict susceptibility to NOR, CIP and GAT by the agar dilution method. The sensitivity and specificity of NAL were 100% and 95%, respectively. Twelve samples were analyzed for mutations in the quinolone resistance-determining region (QRDR) of the gyrA and parC genes....

Co-transmission of Rahnella aquatilis between hospitalized patients

Palavras-chave: Urinary infection; Quinolone resistance; Escherichia coli.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100003

Martins,Willames; Carvalhaes,Cecília Godoy; Cayô,Rodrigo; Gales,Ana Cristina; Pignatari,Antonio Carlos.

ABSTRACT Rahnella aquatilis is an environmental Gram-negative bacillus that is rarely reported as human pathogen, being mainly associated with infections in immunocompromised patients. Herein we describe two cases ofR. aquatilis isolates recovered from endotracheal aspirate cultures of different patients in a tertiary hospital located in the city of São Paulo, Brazil. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rDNA gene sequencing were performed to confirm bacterial identification after the isolates being erroneously identified as Pantoea spp. by automated system. Both isolates showed the same PFGE pattern and presented the ß-lactamase encoding geneblaRAHN-1, responsible for resistance to cephalothin. The isolates...

Early dissemination of OXA-72-producing Acinetobacter baumannii strain in Colombia: a case report

Palavras-chave: Rahnella spp.; Bacteria identification; MALDI-TOF MS; Environmental bacteria.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600648

Saavedra,Sandra Yamile; Cayô,Rodrigo; Gales,Ana Cristina; Leal,Aura Lucia; Saavedra,Carlos Humberto.

Nosocomial infections caused by carbapenem-resistant Acinetobacter baumannii isolates have reached epidemic levels in past decades. Currently this microorganism is responsible for outbreaks of difficult eradication and with high mortality rates worldwide. We herein report a rare case of an OXA-72-producing A. baumannii isolate colonizing a 47-year-old male patient with peritonitis due to abdominal stab wound, four years earlier than the first report of this carbapenemase in Acinetobacter pittii in Colombia. Although OXA-72 presents a low prevalence compared with OXA-23, our study demonstrated that A. baumannii isolates carrying the blaOXA-72 gene were present in the hospital environment in Colombia and could act as a reservoir for further spread to other...

Tipo: Info:eu-repo/semantics/report

Palavras-chave: Acinetobacter baumannii; Carbapenem-resistance; Oxacillinase; Colombia.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600678

Cefepime versus extended spectrum β-lactamase-producing Enterobacteriaceae

Nogueira,Keite da Silva; Daur,Alessandra Vale; Reason,Iara Taborda de Messias; Gales,Ana Cristina; Costa,Libera Maria Dalla.

The objective of this study was to evaluate the susceptibility to cefepime of a large group of ESBL- producing enterobacteria recently isolated in a Brazilian teaching hospital . The study included 280 strains of ESBL-producing enterobacteria, isolated between 2005 and 2008. The presence of the genes blaCTX-M, blaTEM and blaSHV was determined by PCR and confirmed by nucleotide sequencing. Susceptibility testing for cefepime was performed by disc-diffusion, agar dilution method and E-test®. Among the isolates, 34 (12.1%) presented a cefepime inhibition zone > 21 and MIC < 8 mg/L by agar dilution and E-strip methods. The use of cefepime for the treatment of infections caused by ESBL-producing bacteria has been controversial. Some studies of PD/PK...

Antimicrobial activity of doripenem against Gram-negative pathogens: results from INVITA-A-DORI Brazilian study

Palavras-chave: Enterobacteriaceae; Beta-lactamases; Cephalosporin resistance.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200014

Gales,Ana Cristina; Azevedo,Heber D; Cereda,Rosângela Ferraz; Girardello,Raquel; Xavier,Danilo Elias.

In vitro activity of doripenem and comparator antimicrobial agents was evaluated against Gram-negative bacilli recently isolated from Brazilian private hospitals that were enrolled in the INVITA-A-DORI Brazilian Study. A total of 805 unique Gram-negative bacilli were collected from patients hospitalized at 18 medical centers between May/08 and March/09. Each hospital was asked to submit 50 single Gram-negative bacilli isolated from blood, lower respiratory tract or intraabdominal secretions. Bacterial identification was confirmed and antimicrobial susceptibility testing was performed using Clinical Laboratory Standards Institute (CLSI) microdilution method at a central laboratory. CLSI M100-S21 (2011) or US-FDA package insert criteria (tigecycline) was...

Antimicrobial resistance in Enterobacteriaceae in Brazil: focus on β-lactams and polymyxins

Palavras-chave: Carbapenems; Gram-negative bacteria; Brazil.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600003

Sampaio,Jorge Luiz Mello; Gales,Ana Cristina.

ABSTRACT During the last 30 years there has been a dissemination of plasmid-mediated β-lactamases in Enterobacteriaceae in Brazil. Extended spectrum β-lactamases (ESBL) are widely disseminated in the hospital setting and are detected in a lower frequency in the community setting. Cefotaximases are the most frequently detected ESBL type and Klebsiella pneumoniae is the predominant species among ESBL producers. Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae became widely disseminated in Brazil during the last decade and KPC production is currently the most frequent resistance mechanism (96.2%) in carbapenem resistant K. pneumoniae. To date KPC-2 is the only variant reported in Brazil. Polymyxin B resistance in KPC-2-producing K. pneumoniae...

KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil

Palavras-chave: ESBL; KPC; NDM; Extended spectrum β-lactamases; New Delhi metallo-β-lactamase; Klebsiella pneumoniae carbapenemase; Polymyxin B; Colistin; Antimicrobial resistance; Carbapenemases; Brazil; Enterobacteriaceae.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000500031

Biberg,Camila Arguelo; Rodrigues,Ana Claudia Souza; Carmo,Sidiane Ferreira do; Chaves,Claudia Elizabeth Volpe; Gales,Ana Cristina; Chang,Marilene Rodrigues.

The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA...

Antimicrobial susceptibility testing for Helicobacter pylori isolates from Brazilian children and adolescents: comparing agar dilution, E-test, and disk diffusion

Palavras-chave: Klebsiella pneumoniae; Carbapenems; Drug-resistant bacteria.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501

Ogata,Silvio Kazuo; Gales,Ana Cristina; Kawakami,Elisabete.

Antimicrobial susceptibility testing for Helicobacter pylori is increasingly important due to resistance to the most used antimicrobials agents. Only agar dilution method is approved by CLSI, but it is difficult to perform routinely. We evaluated the reliability of E-test and disk diffusion comparing to agar dilution method on Helicobacter pylori antimicrobial susceptibility testing. Susceptibility testing was performed for amoxicillin, clarithromycin, furazolidone, metronidazole and tetracycline using E-test, disk-diffusion and agar dilution method in 77 consecutive Helicobacter pylori strains from dyspeptic children and adolescents. Resistance rates were: amoxicillin - 10.4%, 9% and 68.8%; clarithromycin - 19.5%, 20.8%, 36.3%; metronidazole - 40.2%33.7%,...

Detection and analysis of different interactions between resistance mechanisms and carbapenems in clinical isolates of Klebsiella pneumoniae

Palavras-chave: Helicobacter pylori; E-test; Disk diffusion; Susceptibility test.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400039

Dalmolin,Tanise Vendruscolo; Bianchini,Bianca Vendruscolo; Rossi,Grazielle Guidolin; Ramos,Ana Carolina; Gales,Ana Cristina; Trindade,Priscila de Arruda; Campos,Marli Matiko Anraku de.

Abstract Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Genetic similarity was evaluated by ERIC-PCR. High levels of resistance were verified by the minimum inhibitory concentration for the...