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| Registros recuperados: 15 | |
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| Duval, David; Galinier, Richard; Mouahid, Gabriel; Toulza, Eve; Allienne, Jean Francois; Portela, Julien; Calvayrac, Christophe; Rognon, Anne; Arancibia, Nathalie; Mitta, Guillaume; Theron, Andre; Gourbal, Benjamin. |
| Background Schistosomiasis is the second-most widespread tropical parasitic disease after malaria. Various research strategies and treatment programs for achieving the objective of eradicating schistosomiasis within a decade have been recommended and supported by the World Health Organization. One of these approaches is based on the control of snail vectors in endemic areas. Previous field studies have shown that competitor or predator introduction can reduce snail numbers, but no systematic investigation has ever been conducted to identify snail microbial pathogens and evaluate their molluscicidal effects. Methodology/Principal findings In populations of Biomphalaria glabrata snails experiencing high mortalities, white nodules were visible on snail... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00615/72716/71712.pdf |
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| Tetreau, Guillaume; Dhinaut, Julien; Galinier, Richard; Audant-lacour, Pascaline; Voisin, Sébastien N; Arafah, Karim; Chogne, Manon; Hilliou, Frédérique; Bordes, Anaïs; Sabarly, Camille; Chan, Philippe; Walet-balieu, Marie-laure; Vaudry, David; Duval, David; Bulet, Philippe; Coustau, Christine; Moret, Yannick; Gourbal, Benjamin. |
| In a number of species, individuals exposed to pathogens can mount an immune response and transmit this immunological experience to their offspring, thereby protecting them against persistent threats. Such vertical transfer of immunity, named trans-generational immune priming (TGIP), has been described in both vertebrates and invertebrates. Although increasingly studied during the last decade, the mechanisms underlying TGIP in invertebrates are still elusive, especially those protecting the earliest offspring life stage, i.e. the embryo developing in the egg. In the present study, we combined different proteomic and transcriptomic approaches to determine whether mothers transfer a “signal” (such as fragments of infecting bacteria), mRNA and/or... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00655/76684/77815.pdf |
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| Adema, Coen M.; Hillier, Ladeana W.; Jones, Catherine S.; Loker, Eric S.; Knight, Matty; Minx, Patrick; Oliveira, Guilherme; Raghavan, Nithya; Shedlock, Andrew; Do Amaral, Laurence Rodrigues; Arican-goktas, Halime D.; Assis, Juliana G.; Baba, Elio Hideo; Baron, Olga L.; Bayne, Christopher J.; Bickham-wright, Utibe; Biggar, Kyle K.; Blouin, Michael; Bonning, Bryony C.; Botka, Chris; Bridger, Joanna M.; Buckley, Katherine M.; Buddenborg, Sarah K.; Caldeira, Roberta Lima; Carleton, Julia; Carvalho, Omar S.; Castillo, Maria G.; Chalmers, Iain W.; Christensens, Mikkel; Clifton, Sandra; Cosseau, Celine; Coustau, Christine; Cripps, Richard M.; Cuesta-astroz, Yesid; Cummins, Scott F.; Di Stephano, Leon; Dinguirard, Nathalie; Duval, David; Emrich, Scott; Feschotte, Cedric; Feyereisen, Rene; Fitzgerald, Peter; Fronick, Catrina; Fulton, Lucinda; Galinier, Richard; Gava, Sandra G.; Geusz, Michael; Geyer, Kathrin K.; Giraldo-calderon, Gloria I.; Gomes, Matheus De Souza; Gordy, Michelle A.; Gourbal, Benjamin; Grunau, Christoph; Hanington, Patrick C.; Hoffmann, Karl F.; Hughes, Daniel; Humphries, Judith; Jackson, Daniel J.; Jannotti-passos, Liana K.; Jeremias, Wander De Jesus; Jobling, Susan; Kamel, Bishoy; Kapusta, Aurelie; Kaur, Satwant; Koene, Joris M.; Kohn, Andrea B.; Lawson, Dan; Lawton, Scott P.; Liang, Di; Limpanont, Yanin; Liu, Sijun; Lockyer, Anne E.; Lovato, Tyanna L.; Ludolf, Fernanda; Magrini, Vince; Mcmanus, Donald P.; Medina, Monica; Misra, Milind; Mitta, Guillaume; Mkoji, Gerald M.; Montague, Michael J.; Montelongo, Cesar; Moroz, Leonid L.; Munoz-torres, Monica C.; Niazi, Umar; Noble, Leslie R.; Oliveira, Francislon S.; Pais, Fabiano S.; Papenfuss, Anthony T.; Peace, Rob; Pena, Janeth J.; Pila, Emmanuel A.; Quelais, Titouan; Raney, Brian J.; Rast, Jonathan P.; Rollinson, David; Rosse, Izinara C.; Rotgans, Bronwyn; Routledge, Edwin J.; Ryan, Kathryn M.; Scholte, Larissa L. S.; Storey, Kenneth B.; Swain, Martin; Tennessen, Jacob A.; Tomlinson, Chad; Trujillo, Damian L.; Volpi, Emanuela V.; Walker, Anthony J.; Wang, Tianfang; Wannaporn, Ittiprasert; Warren, Wesley C.; Wu, Xiao-jun; Yoshino, Timothy P.; Yusuf, Mohammed; Zhang, Si-ming; Zhao, Min; Wilson, Richard K.. |
| Biomphalaria snails are instrumental in transmission of the human blood fluke Schistosoma mansoni. With the World Health Organization's goal to eliminate schistosomiasis as a global health problem by 2025, there is now renewed emphasis on snail control. Here, we characterize the genome of Biomphalaria glabrata, a lophotrochozoan protostome, and provide timely and important information on snail biology. We describe aspects of phero-perception, stress responses, immune function and regulation of gene expression that support the persistence of B. glabrata in the field and may define this species as a suitable snail host for S. mansoni. We identify several potential targets for developing novel control measures aimed at reducing snail-mediated transmission of... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00387/49840/71816.pdf |
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| Portet, Anais; Galinier, Richard; Pinaud, Silvain; Portela, Julien; Nowacki, Fanny; Gourbal, Benjamin; Duval, David. |
| Insect thioester-containing protein (iTEP) is the most recently defined group among the thioester-containing protein (TEP) superfamily. TEPs are key components of the immune system, and iTEPs from flies and mosquitoes were shown to be major immune weapons. Initially characterized from insects, TEP genes homologous to iTEP were further described from several other invertebrates including arthropods, cniderians, and mollusks albeit with few functional characterizations. In the freshwater snail Biomphalaria glabrata, a vector of the schistosomiasis disease, the presence of a TEP protein (BgTEP) was previously described in a well-defined immune complex involving snail lectins (fibrinogen-related proteins) and schistosome parasite mucins (SmPoMuc). To... |
| Tipo: Text |
Palavras-chave: Thioester-containing protein; Complement-like protein; Biomphalaria glabrata; Interaction host/pathogens; Innate immunity. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00444/55548/71780.tif |
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| De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume. |
| Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf |
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| Portet, Anais; Pinaud, Silvain; Chaparro, Cristian; Galinier, Richard; Dheilly, Nolwenn M.; Portela, Julien; Charriere, Guillaume; Allienne, Jean-francois; Duval, David; Gourbal, Benjamin. |
| Selective pressures between hosts and their parasites can result in reciprocal evolution or adaptation of specific life history traits. Local adaptation of resident hosts and parasites should lead to increase parasite infectivity/virulence (higher compatibility) when infecting hosts from the same location (in sympatry) than from a foreign location (in allopatry). Analysis of geographic variations in compatibility phenotypes is the most common proxy used to infer local adaptation. However, in some cases, allopatric host-parasite systems demonstrate similar or greater compatibility than in sympatry. In such cases, the potential for local adaptation remains unclear. Here, we study the interaction between Schistosoma and its vector snail Biomphalaria in which... |
| Tipo: Text |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00488/59982/63214.pdf |
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| Pinaud, Silvain; Portela, Julien; Duval, David; Nowacki, Fanny C.; Olive, Marie-aude; Allienne, Jean-francois; Galinier, Richard; Dheilly, Nolwenn M.; Kieffer-jaquinod, Sylvie; Mitta, Guillaume; Theron, Andre; Gourbal, Benjamin. |
| Discoveries made over the past ten years have provided evidence that invertebrate antiparasitic responses may be primed in a sustainable manner, leading to the failure of a secondary encounter with the same pathogen. This phenomenon called "immune priming" or "innate immune memory" was mainly phenomenological. The demonstration of this process remains to be obtained and the underlying mechanisms remain to be discovered and exhaustively tested with rigorous functional and molecular methods, to eliminate all alternative explanations. In order to achieve this ambitious aim, the present study focuses on the Lophotrochozoan snail, Biomphalaria glabrata, in which innate immune memory was recently reported. We provide herein the first evidence that a shift from a... |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00315/42664/71872.pdf |
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| Huot, Camille; Clerissi, Camille; Gourbal, Benjamin; Galinier, Richard; Duval, David; Toulza, Eve. |
| Planorbidae snails are the intermediate host for the trematode parasite of the Schistosoma genus, which is responsible for schistosomiasis, a disease that affects both humans and cattle. The microbiota for Schistosoma has already been described as having an effect on host/parasite interactions, specifically through immunological interactions. Here, we sought to characterize the microbiota composition of seven Planorbidae species and strains. Individual snail microbiota was determined using 16S ribosomal DNA amplicon sequencing. The bacterial composition was highly specific to the host strain with limited interindividual variation. In addition, it displayed complete congruence with host phylogeny, revealing a phylosymbiosis pattern. These results were... |
| Tipo: Text |
Palavras-chave: Microbiota; Phylosymbiosis; Metabarcoding; Planorbid snails; Tripartite interactions; Schistosomiasis. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71807/70295.pdf |
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| Lassalle, Damien; Tetreau, Guillaume; Pinaud, Silvain; Galinier, Richard; Crickmore, Neil; Gourbal, Benjamain; Duval, David. |
| Biomphalaria glabrata is a freshwater Planorbidae snail. In its environment, this mollusk faces numerous microorganisms or pathogens, and has developed sophisticated innate immune mechanisms to survive. The mechanisms of recognition are quite well understood in Biomphalaria glabrata, but immune effectors have been seldom described. In this study, we analyzed a new family of potential immune effectors and characterized five new genes that were named Glabralysins. The five Glabralysin genes showed different genomic structures and the high degree of amino acid identity between the Glabralysins, and the presence of the conserved ETX/MTX2 domain, support the hypothesis that they are pore-forming toxins. In addition, tertiary structure prediction confirms that... |
| Tipo: Text |
Palavras-chave: Pore-forming toxin; Cry toxin; Invertebrate; Biomphalaria glabrata; Host; Pathogen; Vector snail; Innate immunity. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00601/71267/69655.pdf |
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| Delmotte, Jean; Chaparro, Cristian; Galinier, Richard; De Lorgeril, Julien; Petton, Bruno; Stenger, Pierre-louis; Vidal-dupiol, Jeremie; Destoumieux-garzon, Delphine; Gueguen, Yannick; Montagnani, Caroline; Escoubas, Jean Michel; Mitta, Guillaume. |
| Juvenile Pacific oysters (Crassostrea gigas) are subjected to recurrent episodes of mass mortalities that constitute a threat for the oyster industry. This mortality syndrome named “Pacific Oyster Mortality Syndrome” (POMS) is a polymicrobial disease whose pathogenesis is initiated by a primary infection by a variant of an Ostreid herpes virus named OsHV-1 μVar. The characterization of the OsHV-1 genome during different disease outbreaks occurring in different geographic areas has revealed the existence of a genomic diversity for OsHV-1 μVar. However, the biological significance of this diversity is still poorly understood. To go further in understanding the consequences of OsHV-1 diversity on POMS, we challenged five biparental families of oysters to two... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Herpesvirus diversity; Genotype-genotype interactions; Oyster genetic background; Viral populations. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75533/76414.pdf |
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| Duval, David; Pichon, Rémi; Lassalle, Damien; Laffitte, Maud; Gourbal, Benjamain; Galinier, Richard. |
| Thioester-containing proteins (TEPs) superfamily is known to play important innate immune functions in a wide range of animal phyla. TEPs are involved in recognition, and in the direct or mediated killing of several invading organisms or pathogens. While several TEPs have been identified in many invertebrates, only one TEP (named BgTEP) has been previously characterized in the freshwater snail, Biomphalaria glabrata. As the presence of a single member of that family is particularly intriguing, transcriptomic data and the recently published genome were used to explore the presence of other BgTEP related genes in B. glabrata. Ten other TEP members have been reported and classified into different subfamilies: Three complement-like factors (BgC3-1 to BgC3-3),... |
| Tipo: Text |
Palavras-chave: Biomphalaria glabrata; Thioester-containing protein; Alternative splicing; Immunity; Schistosome. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00601/71268/69657.pdf |
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| Pinaud, Silvain; Tetreau, Guillaume; Poteaux, Pierre; Galinier, Richard; Chaparro, Cristian; Lassalle, Damien; Portet, Anaïs; Simphor, Elodie; Gourbal, Benjamin; Duval, David. |
| Aerolysins initially characterized as virulence factors in bacteria are increasingly found in massive genome and transcriptome sequencing data from metazoans. Horizontal gene transfer has been demonstrated as the main way of aerolysin-related toxins acquisition in metazoans. However, only few studies have focused on their potential biological functions in such organisms. Herein, we present an extensive characterization of a multigene family encoding aerolysins - named biomphalysin - in Biomphalaria glabrata snail, the intermediate host of the trematode Schistosoma mansoni. Our results highlight that duplication and domestication of an acquired bacterial toxin gene in the snail genome result in the acquisition of a novel and diversified toxin family.... |
| Tipo: Text |
Palavras-chave: Biomphalaria; Biomphalysin; Aerolysin; Invertebrate immunity; Pore-forming toxin (PFT); Structure. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00687/79909/82814.pdf |
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| Galinier, Richard; Roger, Emmanuel; Mone, Yves; Duval, David; Portet, Anais; Pinaud, Silvain; Chaparro, Cristian; Grunau, Christoph; Genthon, Clemence; Dubois, Emeric; Rognon, Anne; Arancibia, Nathalie; Dejean, Bernard; Theron, Andre; Gourbal, Benjamin; Mitta, Guillaume. |
| In recent decades, numerous studies have sought to better understand the mechanisms underlying the compatibility between Biomphalaria glabrata and Schistosoma mansoni. The developments of comparative transcriptomics, comparative genomics, interactomics and more targeted approaches have enabled researchers to identify a series of candidate genes. However, no molecular comparative work has yet been performed on multiple populations displaying different levels of compatibility. Here, we seek to fill this gap in the literature. We focused on B. glabrata FREPs and S. mansoni SmPoMucs, which were previously demonstrated to be involved in snail/schistosome compatibility. We studied the expression and polymorphisms of these factors in combinations of snail and... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00388/49951/50515.pdf |
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| Portet, Anaïs; Galinier, Richard; Lassalle, Damien; Faille, Alexandre; Gourbal, Benjamin; Duval, David. |
| Biomphalaria glabrata is one of the snail intermediate hosts of Schistosoma mansoni, the causative agent of intestinal schistosomiasis disease. Numerous molecular studies using comparative approaches between susceptible and resistant snails to S. mansoni infection have helped identify numerous snail key candidates supporting such susceptible/resistant status. The functional approach using RNA interference (RNAi) remains crucial to validate the function of such candidates. CRISPR-Cas systems are still under development in many laboratories, and RNA interference remains the best tool to study B. glabrata snail genetics. Herein, we describe the use of modified small interfering RNA (siRNA) molecules to enhance cell delivery, especially into hemocytes, the... |
| Tipo: Text |
Palavras-chave: Biomphalaria glabrata; Cholesteryl TEG; RNAi interference; BgTEP1; Hemocyte. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79376/81902.pdf |
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| Registros recuperados: 15 | |
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