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	Provedor de dados BABT (1) Braz. J. Plant Physiol. (1) Autor Galvão,Rafaelo M. (2) Kobayashi,Adilson K. (2) Bespalhok Filho,João C. (1) Cação,Sandra Maria B. (1) Pereira,Luiz F. P. (1) Pereira,Luiz Filipe Protasio (1) Vieira,Luiz G. E. (1) Vieira,Luiz Gonzaga Esteves (1) Mais... Palavra-chave ACC oxidase (1) Biolistics (1) Climacteric (1) Coffee (1) Ethylene (1) Maturation (1) Transformation (1) Transgenic citrus (1) UidA expression (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Transient gene expression of beta-glucuronidase in citrus thin epicotyl transversal sections using particle bombardment BABT Bespalhok Filho,João C.; Kobayashi,Adilson K.; Pereira,Luiz F. P.; Galvão,Rafaelo M.; Vieira,Luiz G. E.. Studies were carried out to optimize the conditions for transient gene expression through particle bombardment on Carrizo citrange (Citrus sinensis x Poncirus trifoliata) thin epicotyl sections. The best conditions for transient GUS expression were: M-25 tungsten particles, 1550 psi helium pressure, 9 cm distance between specimen and DNA/particle holder and culture of explants in a high osmolarity medium (0.2 M mannitol + 0.2 M sorbitol) 4 h prior and 20 h after bombardment. Under these conditions, an average of 102 blue spots per bombardment (20 explants/plate) were achieved. This protocol is currently being used for transformation of Carrizo citrange and sweet orange (Citrus sinensis). Tipo: Info:eu-repo/semantics/article Palavras-chave: Transformation; UidA expression; Biolistics; Transgenic citrus. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100001 Ethylene production and acc oxidase gene expression during fruit ripening of Coffea arabica L. Braz. J. Plant Physiol. Pereira,Luiz Filipe Protasio; Galvão,Rafaelo M.; Kobayashi,Adilson K.; Cação,Sandra Maria B.; Vieira,Luiz Gonzaga Esteves. The phytohormone ethylene is involved in several physiological and developmental processes in higher plants, including ripening of fruits, abscission of organs and tissues, senescence, wound response as well as in other abiotic stresses. The enzyme 1-aminocyclopropane-1-carboxylate oxidase (ACO) catalyzes the last step of ethylene biosynthesis. The production of ethylene and the expression of a Coffea arabica ACO gene during the last stages of fruit maturation were investigated. A rapid increase of ethylene production at the green-yellow stage of fruit ripening, after the end of endosperm formation, and a decrease of ethylene production at the cherry stage indicates a climacteric phase during ripening. An ACC oxidase (Ca-ACO) from coffee fruit cDNA was... Tipo: Info:eu-repo/semantics/article Palavras-chave: ACC oxidase; Climacteric; Coffee; Ethylene; Maturation. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000300002 Registros recuperados: 2 Primeira ... 1 ... Última

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