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Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis Braz. J. Vet. Res. Anim. Sci.
Cavalcanti,Josemara Neves; Guerra,José Luiz; Gambale,Walderez; Corrêa,Benedito; Paula,Claudete Rodrigues.
Dermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophytosis; Microsporum canis; Ringworm; Guinea pigs.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000500004
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EXPERIMENTAL MODEL OF CANDIDA ALBICANS (SEROTYPES A AND B) ADHERENCE IN VITRO BJM
Pires,Maria de Fátima Costa; Corrêa,Benedito; Gambale,Walderez; Paula,Claudete Rodrigues.
The adherence of Candida albicans is considered an important virulence factor since it determines the colonization of the yeast in various regions of the host, being the initial cause of the infection. In this work we studied the adherence of C. albicans (serotypes A and B) to HeLa and Vero cells after culture for 18 h and 30 h in chemically defined medium (YNB) plus carbohydrate sources such as glucose, sucrose, maltose, galactose and mannose (500 mM). Each assay was performed in triplicate. The best results were obtained with the medium containing glucose. The adherence was greatest in 18 h cultures at 37ºC and at 1 h contact time. The interaction of yeast cells with the host cells was studied by scanning microscopy.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida albicans; Adherence; Serotypes.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300001
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Isolation of Microsporum gypseum in soil samples from different geographical regions of Brazil, evaluation of the extracellular proteolytic enzymes activities (keratinase and elastase) and molecular sequencing of selected strains BJM
Giudice,Mauro Cintra; Reis-Menezes,Adriana Araújo; Rittner,Glauce Mary Gomes; Mota,Adolfo José; Gambale,Walderez.
A survey of Microsporum gypseum was conducted in soil samples in different geographical regions of Brazil. The isolation of dermatophyte from soil samples was performed by hair baiting technique and the species were identified by morphology studies. We analyzed 692 soil samples and the recuperating rate was 19.2%. The activities of keratinase and elastase were quantitatively performed in 138 samples. The sequencing of the ITS region of rDNA was performed in representatives samples. M. gypseum isolates showed significant quantitative differences in the expression of both keratinase and elastase, but no significant correlation was observed between these enzymes. The sequencing of the representative samples revealed the presence of two teleomorphic species of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsporum gypseum; Brazil; Elastase; Keratinase; Sequencing.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300007
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Microbiota indígena do meato acústico externo de gatos hígidos Ciência Rural
Amaral,Ricardo Coutinho do; Ibañez,José Fernando; Mamizuka,Elsa Masae; Gambale,Walderez; Paula,Claudete Rodrigues de; Larsson,Carlos Eduardo.
Cinqüenta felinos hígidos, adultos - vinte e seis machos e vinte e quatro fêmeas - sem definição racial, com distintas idades, reunidos em dois grupos (GRUPO 1- animais domiciliados e GRUPO 2 - animais que rendados) de vinte e cinco animais cada. Após anamnese e prévia sedação, os gatos foram submetidos a exame físico e à otoscopia, comprovando-se a ausência de lesões meatais e timpânicas, com cureta de Buck e wragatoas estéreis, foi colhido material meatal, que foi então submetido a exames bacteriológico e micológico. No Grupo 1, foi evidenciado presença de Otodectes cynotis (4% das amostras), em 72% dos casos de bolores e leveduras (Cladosporium sp 66,6%, Malassezia sp 40%, Penicillium sp 33%, Aspergillus sp 33,3%, Rhodotorula sp 20,0%, Mycelia sp 13,3%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbiota; Orelha; Gatos; Felinos.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781998000300015
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