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| Certad, Gabriela; Zahedi, Alireza; Gantois, Nausicaa; Sawant, Manasi; Creusy, Colette; Duval, Erika; Benamrouz-vanneste, Sadia; Ryan, Una; Viscogliosi, Eric. |
| Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be... |
| Tipo: Text |
Palavras-chave: Piscine Cryptosporidium; Edible marine fish; Genetic characterization; 18S rDNA gene; Actin gene; Molecular phylogeny. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00665/77741/79864.pdf |
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| Certad, Gabriela; Follet, Jérôme; Gantois, Nausicaa; Hammouma-ghelboun, Ourida; Guyot, Karine; Benamrouz-vanneste, Sadia; Fréalle, Emilie; Seesao, Yuwalee; Delaire, Baptiste; Creusy, Colette; Even, Gaël; Verrez-bagnis, Veronique; Ryan, Una; Gay, Mélanie; Aliouat-denis, Cécile; Viscogliosi, Eric. |
| Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of... |
| Tipo: Text |
Palavras-chave: Cryptosporidium; Edible marine fish; 18S rRNA gene; Gp60; Molecular epidemiology; Phylogeny; Novel genotypes; European seas. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00497/60864/64198.pdf |
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