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| Xu,Liang; Tao,Xiaojun; Gao,Yucong; Zhang,Wenjie; Meng,Yihan; Li,Cuiyu; Jiang,Mingyue; Xixiang,Ying. |
| ABSTRACT Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Portulaca oleracea L./hydroxydihydrobovolide; Portulaca oleracea L./hydroxydihydrobovolide/cytotoxicity; Portulaca oleracea L./hydroxydihydrobovolide/pharmacokinetics; Hydroxydihydrobovolide/Ultra-high performance liquid chromatography. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200615 |
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| Zhang,Wenjie; Li,Di; Leng,Aijing; Ai,Junjun; Du,Yang; Meng,Yihan; Gao,Yucong; Ying,Xixiang. |
| The aim of the present study was to investigate the tissue distribution and excretion of five components of Portulaca oleracea L. extract (POE) in rat following oral administration. A rapid, sensitive and specific ultra-high performance liquid chromatography (UHPLC) method with puerarin as the internal standard was used for the quantitative analysis of five components of POE, including caffeic acid (CA), p-coumaric acid (p-CA), ferulic acid (FA), quercitrin (QUER) and hesperidin (HP) in rat tissues including the liver, intestine, stomach, muscle, heart, lung, brain, kidney and spleen, urine and feces. The results show that onlyp-CA and FA were found in nearly all tissues with low cumulative ratios, and CA was higher in the intestine and stomach with a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Portulaca oleracea L./pharmacokinetics; Portulaca oleracea L./extract/experimental study; Portulaca oleracea L./extract/ tissue distribution; Portulaca oleracea L./extrato/excretion; Compostos fenólicos; Ultra performance liquid chromatography/quantitative analysis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300643 |
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