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Estimates of DNA damage by the comet assay in the direct-developing frog Eleutherodactylus johnstonei (Anura, Eleutherodactylidae) Genet. Mol. Biol.
Valencia,Laura Carolina; García,Adriana; Ramírez-Pinilla,Martha Patricia; Fuentes,Jorge Luis.
The aim of this study was to use the Comet assay to assess genetic damage in the direct-developing frog Eleutherodactylus johnstonei. A DNA diffusion assay was used to evaluate the effectiveness of alkaline, enzymatic and alkaline/enzymatic treatments for lysing E. johnstonei blood cells and to determine the amount of DNA strand breakage associated with apoptosis and necrosis. Cell sensitivity to the mutagens bleomycin (BLM) and 4-nitroquinoline-1-oxide (4NQO) was also assessed using the Comet assay, as was the assay reproducibility. Alkaline treatment did not lyse the cytoplasmic and nuclear membranes of E. johnstonei blood cells, whereas enzymatic digestion with proteinase K (40 !g/mL) yielded naked nuclei. The contribution of apoptosis and necrosis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bleomycin; Comet assay; DNA diffusion assay; Eleutherodactylus johnstonei; 4-nitroquinoline-1-oxide.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400022
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Ostrácodos (Crustacea, Ostracoda) y carófitos (Chlorophyta, Charales) de la Formación Desencuentro (Mioceno superior), provincia de La Rioja, Argentina Ameghiniana
Cusminsky,Gabriela; García,Adriana; Herbst,Rafael.
Se describen e ilustran los ostrácodos y carófitos de la Formación Desencuentro, provincia de La Rioja, Argentina. La asociación muestra baja diversidad con seis taxones de ostrácodos: Eucypris sp., Darwinula sp., Cyamocytheridea sp., Cyprideis salebrosa hartmanni Ramírez, Limnocythere aff. L. reticulata Sharpe, Limnocythere sp., y dos especies de carófitos: Chara zeylanica s. l. Klein ex Willdenow y Chara musacchioi García sp. nov. El hallazgo de veinte girogonites bien preservados de Chara musacchioi permitió evaluar su variabilidad morfológica, establecer su relación con la especie actual Chara hornemannii Wallman, y arribar a la conclusión de que se trata de una nueva especie. Estos taxones indican un ambiente acuático de aguas tranquilas oligohalinas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ostracoda; Charales; Paleolimnología; Mioceno Superior; Argentina.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0002-70142006000200006
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Evaluation of foliar fungal endophyte diversity and colonization of medicinal plant Luehea divaricata (Martius et Zuccarini) Biol. Res.
Bernardi-Wenzel,Juliana; García,Adriana; Filho,Celso J R; Prioli,Alberto J; Pamphile,João A.
Endophyte microorganisms are organisms that live inside plants without causing any apparent damage to their hosts. Since all plants exhibit endophyte microorganisms, it is believed that mutual association is of great importance in nature. Luehea divaricata (Martius & Zuccarini), known popularly in Brazil as agoita-cavalo, is a big-sized tree with a wide distribution in the country that possesses medicinal qualities for: dysentery, leucorrhea, rheumatism, blennorrhoea, tumors, bronchitis, and depuration. This research aims at isolating and molecularly characterizing fungi isolates from L. divaricata by sequence analysis of ITS1-5.8S-ITS2 rDNA. Further, the colonization of endophyte in the host plant by Light and Scanning Electron Microscopy will...
Tipo: Journal article Palavras-chave: Endophyte fungi; Luehea divaricata; Diversity; RDNA sequencing; Scanning Electron Microscopy.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400001
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Diversity of foliar endophytic fungi from the medicinal plant Sapindus saponaria L . and their localization by scanning electron microscopy Biol. Res.
García,Adriana; Rhoden,Sandro A; Rubin Filho,Celso J; Nakamura,Celso V; Pamphile,João A.
Endophytic fungi inhabit vegetable tissues or organs, without causing them any harm. Endophytes can co-evolve with plant hosts and possess species-specific interactions. They can protect the plant from insect attacks and diseases, and are also able to produce substances of biotechnological interest. In folk medicine, the bark, roots and fruits of Sapindus saponaria is used to produce substances with anxiolytic, astringent, diuretic and expectorant properties, as well as tonics, blood depuratives and cough medicine. This study evaluated the diversity of endophytic fungi present in the leaves of S. saponaria L. and observed the colonization of host plants by endophytes, using light and scanning electron microscopy. We verified that these fungi are found in...
Tipo: Journal article Palavras-chave: Endophyte; Light microscopy; Phylogenetic analysis; SEM.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000200006
Registros recuperados: 4
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