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Determination of trans-10-hydroxy-2-decenoic acid (10-HDA) in royal jelly from São Paulo State, Brazil Ciênc. Tecnol. Aliment.
Garcia-Amoedo,Luis Henrique; Almeida-Muradian,Ligia Bicudo de.
The 10-HDA content in Brazilian samples (São Paulo State) of royal jelly (RJ) was analyzed using an HPLC method based on the work by BLOODWORTH et al. [2]. The chromatographic conditions were: isocratic system, reversed phase C18-H column, auto sampler, diode array UV-VIS detector adjusted to 225nm, mobile phase composed by methanol/water (45:55) at pH= 2.5 adjusted with phosphoric acid; a-naphtol was used as internal standard, and the running time was 30min. By statistical analysis of the results, the 10-HDA contents of the samples analyzed seem to have two ranges: 1.8% and 3% (w/w), that would be useful to qualify the RJ. This is the first data regarding 10-HDA content of Brazilian RJ.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trans-10-hydroxy-2-decenoic acid; 10-HDA; Royal jelly; Beehive product; HPLC analysis.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612003000400012
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Physicochemical analyses indicated to the quality control of royal jelly with honey Ciênc. Tecnol. Aliment.
Pamplona,Lucila Coelho; Azedo,Ricardo A. B.; Oliveira,Karla Cristina L. S.; Garcia-Amoedo,Luis Henrique; Almeida-Muradian,Ligia Bicudo de.
Royal jelly (RJ) is used as a revitalizing tonic. In order to avoid rejection to its acid taste, it is added to honey. There are regulations for honey and for royal jelly separately but not for the mixture. The objective of this work is, therefore, to verify if the same methods used for pure honey quality control can be used for honey mixed with royal jelly and also the presence of RJ through 10-HDA determination. The methods used were: moisture, reducing sugars, apparent sucrose, ash, hydroxymethylfurfural, insoluble solids, diastase activity, acidity and 10-HDA. Samples were prepared by adding 0-100% of RJ in honey. The results showed that the ash method was the only suitable one to all the samples. The acidity analysis (direct titration) was suitable to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Honey; Royal jelly; Analysis; Bee products; Honey with royal jelly; Quality control.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612004000400022
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