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	Provedor de dados 56 (4) Autor Gaspar,A.M.C. (4) Villar,L.M. (3) Lampe,E. (2) Aloise,R. (1) Amado,L.A. (1) Calado,I.A. (1) Melo,M.M.M. (1) Meyer,A. (1) Morais,L.M. (1) Vitral,C.L. (1) Yoshida,C.F.T. (1) Mais... Palavra-chave Hepatitis A virus (2) Molecular epidemiology (2) Animal model (1) Enzyme immunoassay (1) Experimental infection (1) Hepatitis A virus (HAV) (1) Hepatitis viruses (1) Non-human primates (1) PCR (1) Sero-epidemiology (1) Sub-genotype IA (1) Sub-genotype IB (1) Vaccination (1) Virus titration (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Info:eu-repo/semantics/other (1) Ano 2006 (1) 2004 (2) 1998 (1) País Brazil (4) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última In situ enzyme immunoassay for titration of a Brazilian hepatitis A virus strain (HAF-203) 56 Villar,L.M.; Amado,L.A.; Gaspar,A.M.C.. Hepatitis A virus (HAV) replicates relatively slowly in cell culture without a cytopathic effect, a fact that limits the use of tissue culture assays. The radioimmunofocus assay is the standard method for HAV titration, although it is labor intensive and requires the use of radioisotopes. A simple, rapid and objective infectivity assay based on an in situ enzyme immunoassay (EIA) is described here for a Brazilian cell culture-adapted HAV strain (HAF-203). The assay uses a peroxidase-labeled polyclonal antibody to fixed monolayers as an indicator of infection. EIA may be completed within 7 days using serial 5-fold dilutions of the virus, yielding a titer of 5.024 log 50% tissue culture infective dose (TCID50)/ml for HAF-203. This technique had a detection... Tipo: Info:eu-repo/semantics/other Palavras-chave: Hepatitis A virus; Virus titration; Enzyme immunoassay. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700011 The use of non-human primates as animal models for the study of hepatitis viruses 56 Vitral,C.L.; Yoshida,C.F.T.; Gaspar,A.M.C.. Hepatitis viruses belong to different families and have in common a striking hepatotropism and restrictions for propagation in cell culture. The transmissibility of hepatitis is in great part limited to non-human primates. Enterically transmitted hepatitis viruses (hepatitis A virus and hepatitis E virus) can induce hepatitis in a number of Old World and New World monkey species, while the host range of non-human primates susceptible to hepatitis viruses transmitted by the parenteral route (hepatitis B virus, hepatitis C virus and hepatitis delta virus) is restricted to few species of Old World monkeys, especially the chimpanzee. Experimental studies on non-human primates have provided an invaluable source of information regarding the biology and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis viruses; Non-human primates; Animal model; Experimental infection. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000800003 Co-circulation of genotypes IA and IB of hepatitis A virus in Northeast Brazil 56 Villar,L.M.; Morais,L.M.; Aloise,R.; Melo,M.M.M.; Calado,I.A.; Lampe,E.; Gaspar,A.M.C.. The Northeast region is the location of most cases of acute hepatitis A virus (HAV) in Brazil. In the present study, the genotypes of HAV strains from Pernambuco State, one of most populous states in the Northeast region, were characterized. Blood samples positive for anti-HAV IgM from 145 individuals (mean age = 29.1 years), collected during 2002 and 2003, were submitted to nested RT-PCR for amplification of the 5'non-translated region (5'NTR) and VP1/2A regions of the HAV genome. The VP1/2A and 5'NTR regions were amplified in 39 and 21% of the samples, respectively. Nucleotide sequencing was carried out in 46% of VP1/2A and in 53% of 5'NTR isolates. The identity in nucleotide sequence of the VP1/2A region ranged from 93.6 to 100.0%. Phylogenetic analysis... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis A virus; Molecular epidemiology; Sub-genotype IA; Sub-genotype IB. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700004 Genetic variability of hepatitis A virus isolates in Rio de Janeiro: implications for the vaccination of school children 56 Villar,L.M.; Lampe,E.; Meyer,A.; Gaspar,A.M.C.. The epidemiology of hepatitis A virus (HAV) infection is shifting from high to intermediate endemicity in Brazil, resulting in increased numbers of susceptible individuals and a greater potential for the emergence of outbreaks. Universal vaccination against HAV has been recommended for children, but updated sero-epidemiological data are necessary to analyze the level of natural immunity and to identify candidates for preventive measures. In addition, more molecular studies are necessary to characterize the genotypes involved in HAV infections and outbreaks. Sera from 299 school children (5-15 years old) and 25 school staff members, collected during an outbreak of HAV at a rural public school in June 2000, were tested for IgM and total anti-HAV antibodies... Tipo: Info:eu-repo/semantics/article Palavras-chave: Hepatitis A virus (HAV); PCR; Molecular epidemiology; Sero-epidemiology; Vaccination. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001200003 Registros recuperados: 4 Primeira ... 1 ... Última

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